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引用本文:   桑付明, 李鑫, 刘佳. 纳米基因扩增技术及其应用. 分析化学, 2017, 45(11): 1745-1753. doi:  10.11895/j.issn.0253-3820.170097 [复制]

Citation:   SANG Fu-Ming, LI Xin, LIU Jia. Development of Nano-Polymerase Chain Reaction and Its Application. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1745-1753. doi: 10.11895/j.issn.0253-3820.170097 [复制]

纳米基因扩增技术及其应用

通讯作者:  桑付明, sangfuming@hitwh.edu.cn

收稿日期: 2017-02-17

接受日期: 2017-09-04

出版日期: 2017-11-20

基金项目: 本文系国家自然科学基金(No.21407035)、山东省自然科学基金(No.ZR2014BM021)和威海市大学共建项目(No.2014XGJ15)资助

Development of Nano-Polymerase Chain Reaction and Its Application

Corresponding author:  SANG Fu-Ming, sangfuming@hitwh.edu.cn

Received Date:  2017-02-17

Accepted Date:  2017-09-04

Published Date:  2017-11-20

Fund Project:  This works was supported by the National Natural Science Foundation of China (No. 2140703)

聚合酶链式反应(PCR)是20世纪80年代中期发展起来的一种应用广泛的体外DNA扩增技术,但目前该技术仍然存在着一些问题,如特异性差、灵敏度低和假阳性等。近年来,随着纳米科技的发展,一些纳米粒子如金属纳米粒子、碳纳米材料、量子点和纳米金属氧化物等被引入到PCR反应体系中,即纳米基因扩增技术(NanoPCR)。该技术大幅度提高了PCR的扩增效率、选择性、灵敏度和特异性,推动了生物学技术的发展,具有非常重要的理论意义和应用价值。本文综述了近年来纳米基因扩增技术的主要研究进展、反应机理,并探讨了其应用研究。

关键词:   纳米基因扩增技术, 纳米材料, 特异性, 扩增效率, 热启动, 评述
Key words:   NanoPCR, Nanomaterials, Specificity, Amplification efficiency, Hot-start, Review
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纳米基因扩增技术及其应用

桑付明, 李鑫, 刘佳

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