Citation: LI Jing, HAN Shu-Hua. Bifunctional Fluorescence Molecular Probes for Detection of Aluminum(Ⅲ) and pH. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1346-1352. doi: 10.11895/j.issn.0253-3820.170125 [复制]
Bifunctional Fluorescence Molecular Probes for Detection of Aluminum(Ⅲ) and pH
利用荧光素（Fluorescein）对罗丹明6G（Rhodamine 6G）进行修饰，得到荧光分子探针R6G-Flu杂化物。此探针可特异性识别Al3+，检出限可低至10-8 mol/L级；向含有探针分子的溶液中加入Al3+后，溶液的颜色由无色变为粉色，并且在紫外灯下发出绿色荧光，可实现肉眼对10 μmol/L Al3+的定性检测。考察了不同pH值下R6G-Flu的荧光性质。结果表明，此探针还可用于酸性范围（pH 3.00~6.00）和碱性范围（pH 8.00~10.50）内pH值的精确检测。实验结果表明，R6G-Flu是一种可用于Al3+和pH值检测的双功能荧光分子探针。
A fluorescent molecular probe R6G-Flu was prepared by modifying fluorescein onto Rhodamine 6G. The probe could be used to recognize Al3+ specifically, and the detection limit could reach as low as 10-8 mol/L. After addition of Al3+ (10 μmol/L) to the probe, the solution showed a color change from colorless to pink, and green fluorescence was observed under the UV irradiation, which could be perceived by the naked eye. By measuring the fluorescence emission intensity of R6G-Flu at different pH, the probe could also be used to determine pH in acidic pH range (3.00-6.00) and basic pH range (8.00-10.50). The detection results of Al3+ and pH indicated that the R6G-Flu was a dual-functional fluorescent molecular probe.