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引用本文:   罗辉泰, 黄晓兰, 吴惠勤, 张秋炎, 朱志鑫, 黄芳, 林晓珊. QuEChERS-同位素稀释-液相色谱-高分辨飞行时间质谱法高通量筛查化妆品中86种糖皮质激素. 分析化学, 2017, 45(9): 1381-1388. doi:  10.11895/j.issn.0253-3820.170194 [复制]

Citation:   LUO Hui-Tai, HUANG Xiao-Lan, WU Hui-Qin, ZHANG Qiu-Yan, ZHU Zhi-Xin, HUANG Fang, LIN Xiao-Shan. High Throughput Screening of 86 Kinds of Glucocorticoids in Cosmetics Using QuEChERS and Isotope Dilution-Liquid Chromatography Copuled to High Resolution Time-of-Flight Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1381-1388. doi: 10.11895/j.issn.0253-3820.170194 [复制]

QuEChERS-同位素稀释-液相色谱-高分辨飞行时间质谱法高通量筛查化妆品中86种糖皮质激素

通讯作者:  黄晓兰, wenhxl@126.com

收稿日期: 2017-03-27

接受日期: 2017-06-20

出版日期: 2017-09-20

基金项目: 本文系广东省科技计划项目"公益研究与能力建设"专项(No.2014A040401038)和广东省科技计划项目(No.2014B070705001)资助

High Throughput Screening of 86 Kinds of Glucocorticoids in Cosmetics Using QuEChERS and Isotope Dilution-Liquid Chromatography Copuled to High Resolution Time-of-Flight Mass Spectrometry

Corresponding author:  HUANG Xiao-Lan, wenhxl@126.com

Received Date:  2017-03-27

Accepted Date:  2017-06-20

Published Date:  2017-09-20

建立了QuEChERS-同位素稀释-液相色谱-四极杆串联飞行时间质谱同时快速筛查化妆品中86种糖皮质激素(Glucocorticoids,GCs)的高通量方法。样品经乙腈提取,改进的QuEChERS法净化,待测物选用具有多重色谱保留机理的新型色谱柱Poroshell 120 PFP(100 mm×2.1 mm,2.7 μm),以0.2%(V/V)乙酸和乙腈为流动相进行梯度洗脱分离,在电喷雾离子源的正离子模式下建立了一级精确质量数及二级碎片离子质谱图数据库,无需标准品即可完成化妆品中86种GCs的全面筛查与确证。所有待测物在2~200 μg/L浓度范围内线性良好,相关系数均大于0.99,3个添加水平的平均回收率为66.2%~112.8%,相对标准偏差(RSD)为4.6%~13.9%,检出限(LOD,S/N ≥ 3)为0.006-0.015 mg/kg,定量限(LOQ,S/N ≥ 10)为0.02-0.05 mg/kg。本方法简便高效、定性可靠、定量准确,适用于化妆品中非法添加GCs的高通量筛查。

关键词:   QuEChERS, 同位素稀释, 高分辨飞行时间质谱, 化妆品, 糖皮质激素, 高通量筛查
Key words:   QuEChERS, Isotope dilution, High resolution time-of-flight mass spectrometry, Cosmetics, Glucocorticoids, High throughput Screening
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目录

QuEChERS-同位素稀释-液相色谱-高分辨飞行时间质谱法高通量筛查化妆品中86种糖皮质激素

罗辉泰, 黄晓兰, 吴惠勤, 张秋炎, 朱志鑫, 黄芳, 林晓珊

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