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引用本文:   李潇潇, 丁书茂, 杨旭, 袁均林. 噻呋酰胺人工抗原的合成及酶联免疫吸附分析方法的建立. 分析化学, 2018, 46(1): 27-32. doi:  10.11895/j.issn.0253-3820.170206 [复制]

Citation:   LI Xiao-Xiao, DING Shu-Mao, YANG Xu, YUAN Jun-Lin. Synthesis of Thifluzamide Artificial Antigens and Establishment of Enzyme Linked Immunosorbent Assay Detection Method. Chinese Journal of Analytical Chemistry, 2018, 46(1): 27-32. doi: 10.11895/j.issn.0253-3820.170206 [复制]

噻呋酰胺人工抗原的合成及酶联免疫吸附分析方法的建立

通讯作者:  袁均林, yuanjl@mail.ccnu.edu.cn

收稿日期: 2017-03-30

接受日期: 2017-11-01

出版日期: 2018-01-20

基金项目: 本文系国家自然科学基金项目(No.21577045)资助

Synthesis of Thifluzamide Artificial Antigens and Establishment of Enzyme Linked Immunosorbent Assay Detection Method

Corresponding author:  YUAN Jun-Lin, yuanjl@mail.ccnu.edu.cn

Received Date:  2017-03-30

Accepted Date:  2017-11-01

Published Date:  2018-01-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 21577045)

以化合物2-甲基-4-三氟甲基-5-噻唑甲酸(MTCA)为噻呋酰胺的半抗原合成人工抗原,采用活泼酯法合成免疫原MTCA-BSA,分别采用活泼酯法、混合酸酐法和NN'-羰基二咪唑/4-二甲氨基吡啶(CDI/DMAP)法合成3种包被原MTCA-OVA-1、MTCA-OVA-2和MTCA-OVA-3。免疫动物后,最终以包被原MTCA-OVA-3筛选并获得了具有高特异性的噻呋酰胺多克隆抗体,建立了检测噻呋酰胺的间接竞争酶联免疫吸附(ic-ELISA)分析方法。本方法线性检测范围为0.08~10.00 mg/L,半数抑制浓度(IC50)为1.39 mg/L,检出限为0.08 mg/L,对自来水、湖水和小麦中的噻呋酰胺添加回收率在72.0%~128.3%之间,检测结果与HPLC法具有良好的相关性(R2=0.9994)。本研究建立的ic-ELISA方法可用于环境水样与小麦等农产品中噻呋酰胺残留的快速检测。

关键词:   噻呋酰胺, 人工抗原, 多克隆抗体, 酶联免疫吸附分析
Key words:   Thifluzamide, Artificial antigen, Polyclonal antibody, Enzyme linked immunosorbent assay
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噻呋酰胺人工抗原的合成及酶联免疫吸附分析方法的建立

李潇潇, 丁书茂, 杨旭, 袁均林

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