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引用本文:   赵成军, 马雄辉, 李建平. 基于抗原决定基的胰岛素分子印迹电化学传感器. 分析化学, 2017, 45(9): 1360-1366. doi:  10.11895/j.issn.0253-3820.170256 [复制]

Citation:   ZHAO Cheng-Jun, MA Xiong-Hui, Li Jian-Ping. An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1360-1366. doi: 10.11895/j.issn.0253-3820.170256 [复制]

基于抗原决定基的胰岛素分子印迹电化学传感器

通讯作者:  李建平, likianping@263.net

收稿日期: 2017-04-23

接受日期: 2017-07-06

出版日期: 2017-09-20

基金项目: 本文系国家自然科学基金(Nos.21375031,21765006)项目、广西自然科学基金创新研究团队项目(No.2015GXNSFFA139005)和广西高等学校高水平创新团队及卓越学者计划项目(桂教人[2014]49号)资助

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

Corresponding author:  Li Jian-Ping, likianping@263.net

Received Date:  2017-04-23

Accepted Date:  2017-07-06

Published Date:  2017-09-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21375031, 21765006) and the Natural Science Foundation of Guangxi Province, China (No. 2015GXNSFFA139005)

采用抗原决定基法制备了胰岛素电化学分子印迹传感器。以胰岛素C端多肽作为模板分子,定向自组装在Au电极上,以邻苯二胺为功能单体,电化学聚合制备分子印迹聚合膜。以NaOH为洗脱液,洗脱模板分子,形成的与胰岛素C端多肽三维结构相匹配的分子印迹孔穴能特异性识别胰岛素。重吸附胰岛素分子后,以K3[Fe(CN)6]/K4[Fe(CN)6]为探针,通过测量探针在电极表面产生的电流大小实现胰岛素的间接测定。在1.0×10-14~5.0×10-13 mol/L浓度范围内,传感器的电流响应值与胰岛素浓度呈良好的线性关系,检出限为7.24×10-15 mol/L(3σ)。此传感器具有较好的选择性和稳定性,并成功用于血清样品中胰岛素的测定。

关键词:   胰岛素, 分子印迹, 电化学传感器, 抗原决定基法
Key words:   Insulin, Molecular imprinting, Electrochemical sensor, Epitope imprinting
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基于抗原决定基的胰岛素分子印迹电化学传感器

赵成军, 马雄辉, 李建平

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