Citation: SI Xiao-Xi, ZHU Qin, ZHU Rui-Zhi, WANG Hong-Bo, LIU Qiang, ZHANG Feng-Mei, XIANG Neng-Jun, ZHAO Le, MIAO Ming-Ming, LIU Zhi-Hua. Particle Size Distribution of Phenols in Mainstream Cigarette Smoke Aerosol. Chinese Journal of Analytical Chemistry, 2018, 46(2): 293-299. doi: 10.11895/j.issn.0253-3820.170295 [复制]
Particle Size Distribution of Phenols in Mainstream Cigarette Smoke Aerosol
为研究酚类物质在卷烟主流烟气气溶胶中的粒径分布，采用单通道吸烟机-电子低压撞击器（ELPI），通过12级聚酯薄膜捕集烟气气溶胶粒相物，采用超高效液相色谱-荧光检测方法测定了14种酚类在不同粒径气溶胶中的分布。实验结果表明，本方法捕集得到气溶胶粒相物质量的相对标准偏差小于10%，具有较好的稳定性；超高效液相色谱-荧光检测方法测定14种酚类的线性相关系数R2均大于0.9959，检出限低于1.2 ng/cig，回收率在80.1%-115.0%之间，方法简单快速，准确可靠。采用本方法研究了卷烟主流烟气气溶胶中14种酚类物质含量和浓度的粒径分布，发现除了4-乙基愈创木酚在捕集的气溶胶中未检出外，其它13种酚类物质在不同粒径气溶胶粒相物中的含量分布随粒径增加呈现先增加后减小的趋势，与粒相物质量分布一致，并主要集中在中等粒径（0.261~0.722 μm）的粒相物中，在粒径0.431 μm的粒相物中含量最高；不同酚类物质在不同粒径气溶胶中的浓度分布趋势有差异，苯酚和单取代苯酚类的浓度分布有特异性，其浓度随着粒径增加呈现先增加后减小的趋势，在中等粒径的粒相物中（0.261~0.722 μm）浓度最高；苯二酚类和单取代苯二酚的浓度在0.144~1.166 μm的粒相物中分布无明显差异，二取代苯酚类的浓度随着粒径的变化无明显差异。
To measure particle size distribution of phenols in mainstream cigarette smoke aerosol, the particles of cigarette smoke aerosol were divided into 12 stages using single channel smoking machine coupled electrical low-pressure impactor (ELPI) and collected by 12 polyester films. The collected particles were weighted and then analyzed by ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) to determine the 14 phenols in the different size particles. The results showed that the aerosols collecting method had good stability with relative standard deviation (RSD) of collected particles mass less than 10%. The analyzing results of 14 phenols by UPLC-FLD showed that the linear correlation coefficients (R2) were greater than 0.9959, with detection limits were less than 1.2 ng/cig and recoveries were 80.1%-115.0%. The distributions of 14 phenols with respect to smoke aerosol particle size were investigated. The results indicated that except 4-ethyl-2-methoxy-phenol not detected, the other 13 phenols were detected in mainstream cigarette smoke aerosol. The content of 13 phenols appeared increasing at first and then decreasing with increase of the particle size which distributed in a pattern similar to that of particle mass. All of 13 phenols were present in higher amounts in the medium size particles (0.261-0.722 μm) with peak content in particles 0.431 μm. The distribution of concentrations (ratio of content to particle mass) of 13 phenols in different size particles was different. The concentrations of phenol and mono-substituted phenol appeared to first increase and then decrease with increasing smoke aerosol particle size and were higher in medium size particles (0.261-0.772 μm). The concentrations of benzenediol and mono-substituted benzenediol were uniformly distributed in medium size particles (0.144-1.166 μm), and the concentration of disubstituted phenol was uniform throughout the particles of varying sizes.
Rodgman A, Perfetti T A. The Chemical Components of Tobacco and Tobacco Smoke. CRC press, 2013
Stober W. Rec. Adv. Tob. Sci., 1982, 8: 3-41
YC/T 255-2008, Cigarettes-Determination of Major Phenolic Compounds in Mainstream Cigarette Smoke-High Performance Liquid Chromatographic Method. Tobacco Industry Standards of the People's Republic of China
卷烟主流烟气中主要酚类化合物的测定高效液相色谱法. 中华人民共和国烟草行业标准. YC/T 255-2008