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引用本文:   丁乔棋, 李丽, 范文韬, 吕亚楠, 胡建华, 闫丽萍, 宋素泉. 基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用. 分析化学, 2017, 45(11): 1686-1693. doi:  10.11895/j.issn.0253-3820.170300 [复制]

Citation:   DING Qiao-Qi, LI Li, FAN Wen-Tao, LYU Ya-Nan, HU Jian-Hua, YAN Li-Ping, SONG Su-Quan. Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1686-1693. doi: 10.11895/j.issn.0253-3820.170300 [复制]

基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

通讯作者:  闫丽萍, yanliping@njau.edu.cn; 宋素泉, suquan.song@njau.edu.cn

收稿日期: 2017-05-09

接受日期: 2017-09-04

出版日期: 2017-11-20

基金项目: 本文系国家重点研发计划(Nos.2016YFD0501200,2016YFD0501009)、中央高校基本业务费(No.Y0201500195)、江苏省自然基金面上项目(No.BK20161452)、广东省科技计划重点项目(No.2017B020202010)和江苏高校优势学科建设工程资助项目资助

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

Corresponding author:  YAN Li-Ping, yanliping@njau.edu.cn; SONG Su-Quan, suquan.song@njau.edu.cn

Received Date:  2017-05-09

Accepted Date:  2017-09-04

Published Date:  2017-11-20

Fund Project:  This work was supported by the National Key R&D Program (Nos. 2016YFD0501200, 2016YFD0501009), the Fundamental Research Funds for the Central Universities (No. Y0201500195), the Natural Science Foundation of Jiangsu Province (No. BK20161452), the Key Program of Science and Technology Planning of Guangdong Province, China (No. 2017B020202010), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China)

以羧基化CdTe/ZnSe量子点荧光微球为标记物,通过1-乙基-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)活化法将氯霉素(CAP)单克隆抗体与量子点荧光微球偶联制备荧光探针。氯霉素全抗原(CAP-HS-BSA)及羊抗鼠二抗分别喷涂硝酸纤维素膜,形成检测线(T线)和质控线(C线),组装成新型氯霉素量子点荧光微球免疫层析试纸条,建立了快速、定量检测牛奶中CAP的方法。本研究开发的量子点荧光微球试纸条可在15 min内完成牛奶样品中CAP的定量检测,线性范围为0.1~100.0μg/L,检出限为0.1μg/L。牛奶样品CAP的加标回收率为93.3%~97.9%,相对标准偏差在4.9%~6.9%之间。

关键词:   氯霉素, 量子点微球, 免疫层析试纸条
Key words:   Chloramphenicol, Quantum dot submicrobeads, Immunochromatographic test strip
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目录

基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

丁乔棋, 李丽, 范文韬, 吕亚楠, 胡建华, 闫丽萍, 宋素泉

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