Citation: DING Qiao-Qi, LI Li, FAN Wen-Tao, LYU Ya-Nan, HU Jian-Hua, YAN Li-Ping, SONG Su-Quan. Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1686-1693. doi: 10.11895/j.issn.0253-3820.170300 [复制]
Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol
A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0.1 μg/L, with a working range of 0.1-100 μg/L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93.3% to 97.9% with relative standard deviations of less than 7%.
WU Li-Yuan.The Chinese Livestock and Poultry Breeding, 2014, 10(4):43-44.
吴丽媛. 中国畜禽种业, 2014, 10(4): 43-44