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引用本文:   万传玲, 薛蓉, 湛游洋, 李晓晶, 裴奉奎. N-乙酰半胱氨酸保护下给药钆-二乙三胺五乙酸后慢性肾衰大鼠的尿液代谢组学研究. 分析化学, 2017, 45(9): 1271-1277. doi:  10.11895/j.issn.0253-3820.170307 [复制]

Citation:   WAN Chuan-Ling, XUE Rong, ZHAN You-Yang, LI Xiao-Jing, PEI Feng-Kui. Urinary Metabonomic Study on N-Acetylcysteine-Protected Rats with Chronic Renal Failure from Gd-Diethylenetriamine Pentaacetic Acid Administration. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1271-1277. doi: 10.11895/j.issn.0253-3820.170307 [复制]

N-乙酰半胱氨酸保护下给药钆-二乙三胺五乙酸后慢性肾衰大鼠的尿液代谢组学研究

通讯作者:  李晓晶, xjli@ciac.ac.cn

收稿日期: 2017-04-17

接受日期: 2017-06-20

出版日期: 2017-09-20

基金项目: 本文系国家自然科学基金项目(No.21305134)资助

Urinary Metabonomic Study on N-Acetylcysteine-Protected Rats with Chronic Renal Failure from Gd-Diethylenetriamine Pentaacetic Acid Administration

Corresponding author:  LI Xiao-Jing, xjli@ciac.ac.cn

Received Date:  2017-04-17

Accepted Date:  2017-06-20

Published Date:  2017-09-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (No.21305134)

N-乙酰半胱氨酸(NAC)有减轻造影剂引发肾损伤的作用,但其作用机制尚未明确。本研究采用基于1H NMR的代谢组学方法,结合正交偏最小二乘法判别分析(OPLS-DA),在NAC保护下对慢性肾衰大鼠给药造影剂钆-二乙三胺五乙酸(Gd-DTPA),通过分析大鼠尿液中内源性代谢物的变化,研究了NAC对慢性肾衰大鼠的保护机制。结果表明,慢性肾衰大鼠能量代谢、尿素循环等代谢通路发生紊乱。给药Gd-DTPA后,大鼠尿液中胆碱、N-氧三甲胺、邻羟基苯乙酸苯酯、对羟基苯乙酸苯酯、马尿酸、甘氨酸、烟酸、牛磺酸减少,尿囊素增加;而在NAC保护下相关代谢产物向模型组的恢复,说明NAC对Gd-DTPA引发的大鼠肠道细菌代谢、肝线粒体代谢、犬尿氨酸代谢紊乱及氧化损伤具有一定修复作用。NAC对尿素循环代谢的改善可能减轻大鼠体内的肾损伤,而其对细胞中谷胱甘肽的补充可能减轻Gd-DTPA造成的氧化损伤。

关键词:   代谢组学, 核磁共振, 慢性肾衰, 钆-二乙三胺五乙酸, N-乙酰半胱氨酸
Key words:   Metabonomics, Nuclear magnetic resonance, Chronic renal failure, Gd-diethylenetriamine pentaacetic acid, N-Acetylcysteine
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N-乙酰半胱氨酸保护下给药钆-二乙三胺五乙酸后慢性肾衰大鼠的尿液代谢组学研究

万传玲, 薛蓉, 湛游洋, 李晓晶, 裴奉奎

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