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引用本文:   徐潇颖, 刘柱, 朱炳祺, 梁晶晶, 陈万勤, 罗金文. 在线固相萃取-超高效液相色谱-线性离子阱串联质谱法直接测定水中10种藻类毒素. 分析化学, 2017, 45(11): 1703-1710. doi:  10.11895/j.issn.0253-3820.170339 [复制]

Citation:   XU Xiao-Ying, LIU Zhu, ZHU Bing-Qi, LIANG Jing-Jing, CHEN Wan-Qin, LUO Jin-Wen. Simultaneous Determination of 10 Kinds of Algal Toxins in Water Samples by Online Solid Phase Extration Coupled with Ultra High Performance Liquid Chromatography-Quadrupole Linear Ion Trap Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1703-1710. doi: 10.11895/j.issn.0253-3820.170339 [复制]

在线固相萃取-超高效液相色谱-线性离子阱串联质谱法直接测定水中10种藻类毒素

通讯作者:  罗金文, luojw31@163.com

收稿日期: 2017-05-27

接受日期: 2017-08-29

出版日期: 2017-11-20

基金项目: 本文系浙江省食品药品监管系统科技计划项目(No.SP201704)资助

Simultaneous Determination of 10 Kinds of Algal Toxins in Water Samples by Online Solid Phase Extration Coupled with Ultra High Performance Liquid Chromatography-Quadrupole Linear Ion Trap Mass Spectrometry

Corresponding author:  LUO Jin-Wen, luojw31@163.com

Received Date:  2017-05-27

Accepted Date:  2017-08-29

Published Date:  2017-11-20

建立了全自动在线固相萃取-超高效液相色谱-线性离子阱串联质谱法直接测定水中10种藻类毒素的方法。利用程序实现多次进样,通过在线固相萃取对藻类毒素进行富集,然后切换六通阀,将富集的目标物冲洗至分析柱进行分离后,进入线性离子阱质谱检测。10种藻类毒素在相应的浓度范围内线性关系良好,相关系数均大于0.99,检出限在0.0015~0.0050μg/L之间,3个浓度水平(0.02、0.10和1.00μg/L)的加标回收率为83.7%~98.5%。结果表明,在线固相萃取极大简化了前处理过程,线性离子阱串联质谱法提高了痕量藻类毒素测定的灵敏度,增强子离子扫描(EPI)谱库的建立为藻类毒素的确证提供保障。本方法适用于水体中多种藻类毒素的快速确证和定量测定。

关键词:   藻类毒素, 在线固相萃取, 超高效液相色谱-线性离子阱串联质谱, 水样
Key words:   Algal toxins, Online-solid phase extraction, Ultra performance liquid chromatography-quadrupole linear ion trap mass spectrometry, Water
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在线固相萃取-超高效液相色谱-线性离子阱串联质谱法直接测定水中10种藻类毒素

徐潇颖, 刘柱, 朱炳祺, 梁晶晶, 陈万勤, 罗金文

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