蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用

引用本文: 彭涛, 王见一, 谢三磊, 姚凯, 孙淑娟, 曾于洋, 江海洋. 蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用. 分析化学, 2018, 46(3): 373-378. doi: 10.11895/j.issn.0253-3820.170353 [复制]

Citation: PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang. Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion. Chinese Journal of Analytical Chemistry, 2018, 46(3): 373-378. doi: 10.11895/j.issn.0253-3820.170353 [复制]

蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用

彭涛, 王见一, 谢三磊, 姚凯, 孙淑娟, 曾于洋, 江海洋

中国农业大学动物医学院, 北京 100193

通讯作者: 江海洋, haiyang@cau.edu.cn

收稿日期: 2017-06-05

接受日期: 2018-01-19

出版日期: 2018-03-01

基金项目: 本文系国家自然科学基金项目（No.31672600）和十三五重点专项（No.2017YFF0211003）资助

Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion

PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang

College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Corresponding author: JIANG Hai-Yang, haiyang@cau.edu.cn

Received Date: 2017-06-05

Accepted Date: 2018-01-19

Published Date: 2018-03-01

Fund Project: This work was supported by the National Natural Science Foundation (No. 31672600) and the National keypoint Research and Invention Program during the 13th Five-year Plan Period (No. 2017YFF0211003)

以牛血清白蛋白为稳定剂和还原剂，采用一步法合成荧光金纳米簇，并对其进行了表征。制备的荧光金纳米簇呈较规则的球形，粒径均一，约为（2.00 ±0.05）nm，在紫外灯下发出明显的红色荧光，最大激发波长和发射波长分别为360和635 nm。Hg²⁺可与制备的荧光金纳米簇特异性结合而使其荧光猝灭，基于此建立了"turn-off"型的荧光光谱法快速检测Hg²⁺含量。优化了荧光金纳米簇的用量、pH值、检测体系等条件。荧光强度与Hg²⁺浓度有良好的线性关系，在0.5~75.0 μg/L范围内的线性方程为y=-26.76lgx+803.1（R²=0.9951），在75~900 μg/L浓度范围内的线性方程为y=-0.27x+762.02（R²=0.9959），检出限为0.14 μg/L（3σ）。在最优条件下，本方法可在3 min内完成检测。可快速、灵敏、简便地检测自来水中的Hg²⁺，自来水样品加标回收率在86.8%~113.4%之间（n=3），相对标准偏差小于15%。

关键词: 牛血清白蛋白, 荧光金纳米簇, 汞离子, 自来水

One-step green synthetic approach, with bovine serum albumin (BSA) as stabilizer and reductant, was developed for preparation of BSA hybrid fluorescence gold nanoclusters (AuNCs@BSA). The prepared AuNCs@BSA exhibited strong red fluorescence under UV light illumination. Upon excited at 360 nm, the fluorescence spectrum of AuNCs@BSA exhibited maximum emission peak at 635 nm. AuNCs@BSA was presented as uniform spherical morphology with diameter at (2.0±0.05) nm. The fluorescence of AuNCs@BSA could be quenched by Hg²⁺ because of its metallophilic reaction. Based on the fluorescent spectrometry, a rapid detection system was developed for Hg²⁺ detection in tap water. The AuNCs@BSA amount, pH and buffer system were optimized in this study. According to optimization results, ultrapure water (pH 5.0) was selected to dilute the AuNCs@BSA by 100 times, and 50 μL/well of AuNCs@BSA dilution was applied to detect mercury ion in tap water. Under the optimized conditions, the detection could be completed within 3 min, the fluorescence intensity of the system was linearly proportional to the concentration of mercury ion in the range of 0.5-900 μg/L with linear equations y=-26.76lgx + 803.1 (0.5-75 μg/L, R²=0.9951) and y=-0.27x+762.02 (75-900 μg/L, R²=0.9959). The limit of detection was 0.14 μg/L(3σ). The average recoveries in spiked tape water samples ranged from 86.8%-113.4% with relative standard deviation of less than 15%. The result implied that the developed method was able to apply to detect mercury ion rapidly, sensitively and conveniently.

Key words: Bovine serum albumin, Fluorescence gold nanoclusters, Mercury ion, Tap water.

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研究报告

蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用

彭涛, 王见一, 谢三磊, 姚凯, 孙淑娟, 曾于洋, 江海洋

关键词: 牛血清白蛋白, 荧光金纳米簇, 汞离子, 自来水

Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion

PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang

Key words: Bovine serum albumin, Fluorescence gold nanoclusters, Mercury ion, Tap water.

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