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引用本文:   彭涛, 王见一, 谢三磊, 姚凯, 孙淑娟, 曾于洋, 江海洋. 蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用. 分析化学, 2018, 46(3): 373-378. doi:  10.11895/j.issn.0253-3820.170353 [复制]

Citation:   PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang. Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion. Chinese Journal of Analytical Chemistry, 2018, 46(3): 373-378. doi: 10.11895/j.issn.0253-3820.170353 [复制]

蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用

通讯作者:  江海洋, haiyang@cau.edu.cn

收稿日期: 2017-06-05

接受日期: 2018-01-19

出版日期: 2018-03-01

基金项目: 本文系国家自然科学基金项目(No.31672600)和十三五重点专项(No.2017YFF0211003)资助

Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion

Corresponding author:  JIANG Hai-Yang, haiyang@cau.edu.cn

Received Date:  2017-06-05

Accepted Date:  2018-01-19

Published Date:  2018-03-01

Fund Project:  This work was supported by the National Natural Science Foundation (No. 31672600) and the National keypoint Research and Invention Program during the 13th Five-year Plan Period (No. 2017YFF0211003)

以牛血清白蛋白为稳定剂和还原剂,采用一步法合成荧光金纳米簇,并对其进行了表征。制备的荧光金纳米簇呈较规则的球形,粒径均一,约为(2.00 ±0.05)nm,在紫外灯下发出明显的红色荧光,最大激发波长和发射波长分别为360和635 nm。Hg2+可与制备的荧光金纳米簇特异性结合而使其荧光猝灭,基于此建立了"turn-off"型的荧光光谱法快速检测Hg2+含量。优化了荧光金纳米簇的用量、pH值、检测体系等条件。荧光强度与Hg2+浓度有良好的线性关系,在0.5~75.0 μg/L范围内的线性方程为y=-26.76lgx+803.1(R2=0.9951),在75~900 μg/L浓度范围内的线性方程为y=-0.27x+762.02(R2=0.9959),检出限为0.14 μg/L(3σ)。在最优条件下,本方法可在3 min内完成检测。可快速、灵敏、简便地检测自来水中的Hg2+,自来水样品加标回收率在86.8%~113.4%之间(n=3),相对标准偏差小于15%。

关键词:   牛血清白蛋白, 荧光金纳米簇, 汞离子, 自来水
Key words:   Bovine serum albumin, Fluorescence gold nanoclusters, Mercury ion, Tap water.
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目录

蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用

彭涛, 王见一, 谢三磊, 姚凯, 孙淑娟, 曾于洋, 江海洋

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