Citation: PENG Tao, WANG Jian-Yi, XIE San-Lei, YAO Kai, SUN Shu-Juan, ZENG Yu-Yang, JIANG Hai-Yang. Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion. Chinese Journal of Analytical Chemistry, 2018, 46(3): 373-378. doi: 10.11895/j.issn.0253-3820.170353 [复制]
Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion
以牛血清白蛋白为稳定剂和还原剂，采用一步法合成荧光金纳米簇，并对其进行了表征。制备的荧光金纳米簇呈较规则的球形，粒径均一，约为（2.00 ±0.05）nm，在紫外灯下发出明显的红色荧光，最大激发波长和发射波长分别为360和635 nm。Hg2+可与制备的荧光金纳米簇特异性结合而使其荧光猝灭，基于此建立了"turn-off"型的荧光光谱法快速检测Hg2+含量。优化了荧光金纳米簇的用量、pH值、检测体系等条件。荧光强度与Hg2+浓度有良好的线性关系，在0.5~75.0 μg/L范围内的线性方程为y=-26.76lgx+803.1（R2=0.9951），在75~900 μg/L浓度范围内的线性方程为y=-0.27x+762.02（R2=0.9959），检出限为0.14 μg/L（3σ）。在最优条件下，本方法可在3 min内完成检测。可快速、灵敏、简便地检测自来水中的Hg2+，自来水样品加标回收率在86.8%~113.4%之间（n=3），相对标准偏差小于15%。
One-step green synthetic approach, with bovine serum albumin (BSA) as stabilizer and reductant, was developed for preparation of BSA hybrid fluorescence gold nanoclusters (AuNCs@BSA). The prepared AuNCs@BSA exhibited strong red fluorescence under UV light illumination. Upon excited at 360 nm, the fluorescence spectrum of AuNCs@BSA exhibited maximum emission peak at 635 nm. AuNCs@BSA was presented as uniform spherical morphology with diameter at (2.0±0.05) nm. The fluorescence of AuNCs@BSA could be quenched by Hg2+ because of its metallophilic reaction. Based on the fluorescent spectrometry, a rapid detection system was developed for Hg2+ detection in tap water. The AuNCs@BSA amount, pH and buffer system were optimized in this study. According to optimization results, ultrapure water (pH 5.0) was selected to dilute the AuNCs@BSA by 100 times, and 50 μL/well of AuNCs@BSA dilution was applied to detect mercury ion in tap water. Under the optimized conditions, the detection could be completed within 3 min, the fluorescence intensity of the system was linearly proportional to the concentration of mercury ion in the range of 0.5-900 μg/L with linear equations y=-26.76lgx + 803.1 (0.5-75 μg/L, R2=0.9951) and y=-0.27x+762.02 (75-900 μg/L, R2=0.9959). The limit of detection was 0.14 μg/L(3σ). The average recoveries in spiked tape water samples ranged from 86.8%-113.4% with relative standard deviation of less than 15%. The result implied that the developed method was able to apply to detect mercury ion rapidly, sensitively and conveniently.
GB5749-2006. Sanitary Standard for Drinking Water Quality. National standards of the People's Republic of China
生活饮用水卫生标准. 中华人民共和国国家标准. GB5749-2006