会员登陆: 用户名:  密码: 验证码:
首页 杂志概况 投稿须知 在线投稿 在线阅读 征订启事 广告服务 行业资讯 企业动态 资料中心  专访报道 会展信息 ENGLISH

引用本文:   林冬果, 林锦琼, 李佩文, 杨娜, 徐邦牢, 刘大渔. 开放式微流控芯片上的肿瘤组织微阵列构建. 分析化学, 2018, 46(1): 113-120. doi:  10.11895/j.issn.0253-3820.171046 [复制]

Citation:   LIN Dong-Guo, LIN Jin-Qiong, LI Pei-Wen, YANG Na, XU Bang-Lao, LIU Da-Yu. Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip. Chinese Journal of Analytical Chemistry, 2018, 46(1): 113-120. doi: 10.11895/j.issn.0253-3820.171046 [复制]

开放式微流控芯片上的肿瘤组织微阵列构建

通讯作者:  徐邦牢, banglaoxu@163.com

收稿日期: 2017-06-29

接受日期: 2017-10-18

出版日期: 2018-01-20

基金项目: 本文系国家自然科学基金(Nos.81371649,81171418,81602434)和广州市科创委协同创新项目(Nos.20141A010007,[2016]171)资助

Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip

Corresponding author:  XU Bang-Lao, banglaoxu@163.com

Received Date:  2017-06-29

Accepted Date:  2017-10-18

Published Date:  2018-01-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 81371649, 81171418, 81602434) and the Guangzhou Science Technology and Innovation Comission (Nos. 20141A010007, [2016]171)

构建了一种具有自动形成细胞培养阵列和多步骤灵活操作特点的开放式微流控芯片。此芯片具有3层复合式结构:底层为微通道贯穿的细胞培养池阵列,顶层是开放式培养池,二者之间为一层纳米孔薄膜。纳米孔薄膜具有"透气阻水"的特性,既起到止流阀作用实现液体自动分配,又允许跨膜扩散,模拟血管内皮层扩散屏障结构。结合移液工作站,开放式微流控芯片可以完成细胞换液、药物处理和细胞活力测试等一系列分析步骤。本研究以乳腺癌细胞为模型,在芯片上90 s内可构建包含三维细胞培养和仿真血管内皮的10×10肿瘤组织微阵列。形态学和免疫组织化学检测证实了芯片肿瘤组织的仿真效果。抗肿瘤药物测试结果表明,这种开放式微流控组织阵列芯片允许在仿真条件下进行包含复杂操作步骤的细胞实验,是细胞研究的有利工具。

关键词:   开放式微流控芯片, 纳米孔薄膜, 三维细胞培养, 组织微阵列, 抗肿瘤药物测试
Key words:   Open-access microfluidic chip, Nanoporous membrane, Microenvironment, Tissue microarray, Anti-cancer drug testing
[1]

El-Ali J, Sorger P K. Nature, 2006, 442(7101): 403-411. doi: 10.1038/nature05063

[2]

LI Dong-Shun, XU Yi, PENG Jin-Lan, ZHOU Zhen, GAN Jun, WANG Rao. Chem. J. Chinese Universities, 2012, 33(1): 63-67

李栋顺, 徐溢, 彭金兰, 周桢, 甘俊, 王尧. 高等学校化学学报, 2012, 33(1): 63-67

[3]

Mehling M. Tay S. Curr. Opin. Biotechnol., 2014, 25: 95-102. doi: 10.1016/j.copbio.2013.10.005

[4]

Marimuthu M, Kim S. Anal. Bbiochem., 2011, 413(2): 81-89. doi: 10.1016/j.ab.2011.02.027

[5]

QI Ming-Yue, DU Yan, LIU Wei-Ping, CHEN Bin, LIANG Guang-Tie, XU Bang-Lao, LIU Da-Yu. Chinese J. Anal. Chem., 2016, 44(4): 610-616. doi: 10.11895/j.issn.0253-3820.160035

齐明月, 杜燕, 刘未平, 陈斌, 梁广铁, 徐邦牢, 刘大渔. 分析化学, 2016, 44(4): 610-616. doi: 10.11895/j.issn.0253-3820.160035

[6]

Young E W, Beebe D J. Chem. Soc. Rev., 2010, 39(3): 1036-1048. doi: 10.1039/b909900j

[7]

van Duinen V, Trietsch S J, Joore J, Vulto P, Hankemeier T. Curr. Opin. Biotechnol., 2015, 35: 118-126. doi: 10.1016/j.copbio.2015.05.002

[8]

WANG Wei-Xin, LIU Wei-Ping, WU Bin, LIANG Guang-Tie, LIU Da-Yu. Chinese J. Anal. Chem., 2015, 43(5): 637-642. doi: 10.11895/j.issn.0253-3820.150142

王伟鑫, 刘未平, 吴斌, 梁广铁, 刘大渔. 分析化学, 2015, 43(5): 637-642. doi: 10.11895/j.issn.0253-3820.150142

[9]

Li X J, Valadez A V, Zuo P, Nie Z. Bioanalysis, 2012, 4(12): 1509-1525. doi: 10.4155/bio.12.133

[10]

Inamdar N K, Borenstein J T. Curr. Opin. Biotechnol., 2011, 22(5): 681-689. doi: 10.1016/j.copbio.2011.05.512

[11]

Nalayanda D D, Puleo C M, Fulton W B, Wang T H, Abdullah F. Exp. Lung Res., 2007, 33(6): 321-335. doi: 10.1080/01902140701557754

[12]

Hung P J, Lee P J, Sabounchi P, Lin R, Lee L P. Biotechnol. Bioengineer., 2005, 89(1): 1-8. doi: 10.1002/(ISSN)1097-0290

[13]

Huang S B, Wang S S, Hsieh C H, Lin Y C, Lai C S, Wu M H. Lab Chip, 2013, 13(6): 1133-1143. doi: 10.1039/c2lc41264k

[14]

Huang S B, Wu M H, Wang S S, Lee G B. Biomed. Microdevices, 2011, 13(3): 415-430. doi: 10.1007/s10544-011-9510-1

[15]

Evander M, Johansson L, Lilliehorn T, Piskur J, Lindvall M, Johansson S, Almqvist M, Laurell T, Nilsson J. Anal. Chem., 2007, 79(7): 2984-2991. doi: 10.1021/ac061576v

[16]

Toh Y C, Zhang C, Zhang J, Khong Y M, Chang S, Samper V D, van Noort D, Hutmacher D W, Yu H. Lab Chip, 2007, 7(3): 302-309. doi: 10.1039/b614872g

[17]

Giulitti S, Magrofuoco E, Prevedello L, Elvassore N. Lab Chip, 2013, 13(22): 4430-4441. doi: 10.1039/c3lc50643f

[18]

Goral V N, Hsieh Y C, Petzold O N, Clark J S, Yuen P K, Faris R A. Lab Chip, 2010, 10(24): 3380-3386. doi: 10.1039/c0lc00135j

[19]

Lei K F, Wu M H, Hsu C W, Chen Y D. Biosens. Bioelectron., 2014, 51: 16-21. doi: 10.1016/j.bios.2013.07.031

[20]

Clark A M, Sousa K M, Chisolm C N, MacDougald O A, Kennedy R T. Anal. Bioanal. Chem., 2010, 397(7): 2939-2947. doi: 10.1007/s00216-010-3897-z

[21]

Keenan T M, Frevert C W, Wu A, Wong V, Folch A. Lab Chip, 2010, 10(1): 116-122. doi: 10.1039/B913494H

[22]

Jowhar D, Wright G, Samson P C, Wikswo J P, Janetopoulos C. Integr. Biol., 2010, 2(11-12): 648-658. doi: 10.1039/c0ib00110d

[23]

de Groot T E, Veserat K S, Berthier E, Beebe D J, Theberge A B. Lab Chip, 2016, 16(2): 334-344. doi: 10.1039/C5LC01353D

[24]

Wright G A, Costa L, Terekhov A, Jowhar D, Hofmeister W, Janetopoulos C. Microsc. Microanal., 2012, 18(4): 816-828. doi: 10.1017/S1431927612000475

[25]

Malboubi M, Jayo A, Parsons M, Charras G. Microelectron. Engineer., 2015, 144: 42-45. doi: 10.1016/j.mee.2015.02.022

[26]

Lo J F, Sinkala E, Eddington D T. Lab Chip, 2010, 10(18): 2394-2401. doi: 10.1039/c004660d

[27]

Zhu Y, Zhang Y X, Cai L F, Fang Q. Anal. Chem., 2013, 85(14): 6723-6731. doi: 10.1021/ac4006414

[28]

Du G S, Pan J Z, Zhao S P, Zhu Y, den Toonder J M, Fang Q. Anal. Chem., 2013, 85(14): 6740-6747. doi: 10.1021/ac400688f

[29]

Duffy D C, McDonald J C, Schueller O J, Whitesides G M. Anal. Chem., 1998, 70(23): 4974-4984. doi: 10.1021/ac980656z

[30]

Aran K, Sasso L A, Kamdar N, Zahn J D. Lab Chip, 2010, 10(5): 548-552. doi: 10.1039/b924816a

[31]

Prokop A, Prokop Z, Schaffer D, Kozlov E, Wikswo J, Cliffel D, Baudenbacher F. Biomed. Microdevices, 2004, 6(4): 325-339. doi: 10.1023/B:BMMD.0000048564.37800.d6

计量
  • PDF下载量(11)
  • 文章访问量(96)
  • HTML全文浏览量(1)

目录

开放式微流控芯片上的肿瘤组织微阵列构建

林冬果, 林锦琼, 李佩文, 杨娜, 徐邦牢, 刘大渔

Figures and Tables