Citation: LYU Yang, ZHANG Ya-Wen, TAN Lei, JI Wen-Liang, YU Ping, MAO Lan-Qun, ZHOU Fang. Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1595-1599. doi: 10.11895/j.issn.0253-3820.171091 [复制]
Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model
Acute traumatic spinal cord injury (SCI) represents one of the most devastating injuries that afflict the human body. Ascorbic acid (AA) plays an important role in mammalian central nervous system, especially in SCI. In this study, the change of AA concentration after SCI was investigated by using an on-line electrochemical method integrated with in vivo microdialysis. A microdialysis probe (2 mm in length) was implanted into the spinal cord of an anesthetized rat (Thoracic-10). Microdialysis perfusate (2 μL/min) was collected in the sample loop of an on-line injector for direct injection onto a glassy carbon electrode which was modified with the heat-treated single-walled carbon nanotubes (SWNTs). Normal ascorbic acid concentration in the extracellular fluids of spinal cords was (26.17 ±1.25) μmol/L (n=8). The experimental spinal cord injury, induced by a lesion at T-10, significantly increased the extracellular ascorbic acid levels to (53.24±1.95) μmol/L (n=8). This study provides the experimental evidence on the essential roles of ascorbic acid in spinal cord injuries.