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引用本文:   吕扬, 张雅文, 谭磊, 纪文亮, 于萍, 毛兰群, 周方. 脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析. 分析化学, 2017, 45(11): 1595-1599. doi:  10.11895/j.issn.0253-3820.171091 [复制]

Citation:   LYU Yang, ZHANG Ya-Wen, TAN Lei, JI Wen-Liang, YU Ping, MAO Lan-Qun, ZHOU Fang. Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1595-1599. doi: 10.11895/j.issn.0253-3820.171091 [复制]

脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

通讯作者:  毛兰群, lqmao@iccas.ac.cn; 周方, zhou.md@126.com

收稿日期: 2017-07-13

接受日期: 2017-09-13

出版日期: 2017-11-20

基金项目: 本文系北京市科技计划项目(新)(No.2144000021)资助

Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model

Corresponding author:  MAO Lan-Qun, lqmao@iccas.ac.cn; ZHOU Fang, zhou.md@126.com

Received Date:  2017-07-13

Accepted Date:  2017-09-13

Published Date:  2017-11-20

Fund Project:  This work was supported by the Beijing Science and Technology Project (New), China (No.2144000021)

利用活体微透析技术结合在线高选择性抗坏血酸电化学检测技术,以实验性胸10节段脊髓钳夹损伤为动物模型,研究了脊髓损伤过程中胞外抗坏血酸的变化规律。结果表明,微透析探针植入及钳夹损伤瞬间,均存在脊髓抗坏血酸浓度高峰,随后浓度回到稳定水平,对照组大鼠脊髓细胞外液中抗坏血酸的平均基础浓度为(26.17±1.25)μmol/L(n=8)。实验组脊髓钳夹损伤后,胞外抗坏血酸水平显著增加,达到(53.24±1.95)μmol/L(n=8),这种显著性升高可能与继发性脊髓损伤保护机制导致胞内抗坏血酸外流相关。本研究结果为抗坏血酸参与调节继发性脊髓损伤提供了直接的实验证据。

关键词:   抗坏血酸, 脊髓损伤, 活体微透析, 在线电化学检测
Key words:   Ascorbic acid, Spinal cord injury, In vivo microdialysis, On-line electrochemical detection
[1]

Chehensse C, Bahrami S, Denys P, Clement P, Bernabe J, Giuliano F. Hum. Reprod. Update, 2013, 19(5): 507-526. doi: 10.1093/humupd/dmt029

[2]

Serarslan Y, Yonden Z, Ozgiray E. J. Clin. Neurosci., 2010, 17(3): 349-352. doi: 10.1016/j.jocn.2009.03.036

[3]

Tsai P J, Chen W Y, Tzeng S F, Liang W M, Yang C S. Clin. Chim. Acta, 2005, 362(1-2): 94-100. doi: 10.1016/j.cccn.2005.05.033

[4]

Yusa T. Brain Res., 2001, 897(1-2): 104-113. doi: 10.1016/S0006-8993(01)02099-6

[5]

Szultka M, Buszewska-Forajta M, Kaliszan R, Buszewski B. Electrophoresis, 2014, 35(4): 585-592. doi: 10.1002/elps.201300439

[6]

Jin P, Xia L, Li Z, Che N, Zou D, Hu X. J. Pharm. Biomed. Anal., 2012, 70: 151-157. doi: 10.1016/j.jpba.2012.06.020

[7]

Zhu M, Zeng C, Ye J. Electroanalysis, 2011, 23(4): 907-914. doi: 10.1002/elan.201000712

[8]

Liu K, Yu P, Lin Y, Wang Y, Ohsaka T, Mao L. Anal Chem., 2013, 85(20): 9947-9954. doi: 10.1021/ac402620c

[9]

Li L, Zhang Y, Hao J, Liu J, Yu P, Ma F, Mao L. Analyst, 2016, 141(7): 2199-2207. doi: 10.1039/C6AN00064A

[10]

Tao L, Song C, Sun Y. Anal. Chim. Acta, 2013, 761: 194-200. doi: 10.1016/j.aca.2012.11.046

[11]

Talac R, Friedman J A, Moore M J. Biomaterials, 2004, 25(9): 1505-1510. doi: 10.1016/S0142-9612(03)00497-6

[12]

ZHANG Ren-Yan, ZHANG Xue-Ao, JIA Hong-Hui, LI Xin-Hua. Chinese J. Anal. Chem., 2012, 40(6): 909-914

张仁彦, 张学骜, 贾红辉, 李新华. 分析化学, 2012, 40(6): 909-914

[13]

ZHANG Cui-Zhong, WANG Li-Wei, LU Yong-Ke, ZHENG Guang-Jin, PENG Jin-Yun. Chinese J. Anal. Chem., 2016, 44(8): 1263-1269. doi: 10.11895/j.issn.0253-3820.160095

张翠忠, 王丽伟, 卢永课, 郑广进, 彭金云. 分析化学, 2016, 44(8): 1263-1269. doi: 10.11895/j.issn.0253-3820.160095

[14]

Barabas J, Nagy E, Degrell I. Arch. Gerontol. Geriatr., 1995, 21(1): 43-48. doi: 10.1016/0167-4943(95)00654-4

[15]

Liu D, Xu G Y, Pan E, McAdoo D J. Neuroscience, 1999, 93(4): 1383-1389. doi: 10.1016/S0306-4522(99)00278-X

[16]

McAdoo D J, Xu G Y, Robak G, Hughes M G. Exp. Neurol., 1999, 159(2): 538-544. doi: 10.1006/exnr.1999.7166

[17]

Xu G Y, Hughes M G, Ye Z, Hulsebosch C E, McAdoo D J. Exp. Neurol., 2004, 187(2): 329-336. doi: 10.1016/j.expneurol.2004.01.029

[18]

McAdoo D J, Wu P. Pharmacol. Biochem. Behav., 2008, 90(2): 282-296. doi: 10.1016/j.pbb.2008.03.001

[19]

Thompson R J, Zhou N, MacVicar B A. Science, 2006, 312(5775): 924-927. doi: 10.1126/science.1126241

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脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

吕扬, 张雅文, 谭磊, 纪文亮, 于萍, 毛兰群, 周方

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