引用本文: 刘红文, 季颖, 宋春丽, 田刚, 李诗雅, 杨桂英, 吕昌银. 铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法. 分析化学, 2018, 46(5): 658-663. doi: 10.11895/j.issn.0253-3820.171151 [复制]
Citation: LIU Hong-Wen, JI Ying, SONG Chun-Li, TIAN Gang, LI Shi-Ya, YANG Gui-Ying, LYU Chang-Yin. Pb2+-Induced Allosteric PS2.M as A Label-Free Colorimetric Sensor for Detection of Cumulative Radon Radiation. Chinese Journal of Analytical Chemistry, 2018, 46(5): 658-663. doi: 10.11895/j.issn.0253-3820.171151 [复制]
铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法
Pb2+-Induced Allosteric PS2.M as A Label-Free Colorimetric Sensor for Detection of Cumulative Radon Radiation
以氡辐射衰变最终形成稳定的子体铅作为目标检测物，建立了检测氡累积浓度的核酸适配体生物分析新方法。K+诱导富G单链核苷酸PS2.M形成反平行G-四链体四链体，辅因子血红素与G-四联链体结合，形成具有过氧化物酶活性的稳定复合物，催化TMB-H2O2体系发生显色反应。同时加入Pb2+时，Pb2+诱导PS2.M形成结构更加稳定的"缺口"型反平行G-四链体，K+和血红素游离在溶液之中，体系吸光度响应值急剧降低。基于此构建了一种基于Pb2+诱导PS2.M构象改变的过氧化物酶活性"Turn-off"型比色传感器。在5.0×10-9~1.8×10-7 mol/L范围内，吸光度降低值ΔA与Pb2+浓度呈线性关系，线性回归方程为ΔA=0.36+0.13C，R=0.9987。本方法对Pb2+的检出限为3.76 nmol/L（S/N=3），氡的检出限为1.96×103 Bq·h/m3（S/N=3）。本方法灵敏、准确，样品溶液可直接检测，操作简单，避免了现场进行氡辐射剂量测量的放射性危害，为环境中氡的检测提供了新方法。
A new sensitive method for detection of cumulative radon radiation based on the lead was established. PS2.M which stabilized by K+ with hemin as a co-factor exhibits superior peroxidase-like activity, and can effectively catalyze the H2O2-mediated oxidation of TMB. In the presence of Pb2+, K+-stabilized PS2.M DNAzymes are induced to undergo a conformational change, because Pb2+ has a higher efficiency with regard to stabilizing G-quadruplexes than K+, accompanied by a decrease of catalytic activity and a sharp decrease of readout signal. In the work, a novel "turn-off" model of colorimetry-Pb2+ biosensor based on superior peroxidase-like activity of G-quadruplex for Pb2+ analysis was developed. The fading degree of reaction system (ΔA value) was linearly related to Pb2+ concentration in the range of 5.0×10-9-1.8×10-7 mol/L. The linear regression equation was ΔA=0.36+0.13C (10-8 mol/L), with R=0.9987. The detection limits of lead and radon were 3.76 nmol/L(S/N=3) and 1.96×103 Bq·h/m3(S/N=3), respectively. The method exhibited good selectivity, high sensitivity and convenient operation, and could avoid the radioactive hazard in determination of the radon in environment.
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铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法
Pb2+-Induced Allosteric PS2.M as A Label-Free Colorimetric Sensor for Detection of Cumulative Radon Radiation
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