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引用本文:   刘红文, 季颖, 宋春丽, 田刚, 李诗雅, 杨桂英, 吕昌银. 铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法. 分析化学, 2018, 46(5): 658-663. doi:  10.11895/j.issn.0253-3820.171151 [复制]

Citation:   LIU Hong-Wen, JI Ying, SONG Chun-Li, TIAN Gang, LI Shi-Ya, YANG Gui-Ying, LYU Chang-Yin. Pb2+-Induced Allosteric PS2.M as A Label-Free Colorimetric Sensor for Detection of Cumulative Radon Radiation. Chinese Journal of Analytical Chemistry, 2018, 46(5): 658-663. doi: 10.11895/j.issn.0253-3820.171151 [复制]

铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法

通讯作者:  吕昌银, lchy1955@163.com

收稿日期: 2017-08-05

接受日期: 2018-03-31

出版日期: 2018-05-20

基金项目: 本文系国家自然科学基金项目(No.81473021)和湖南省研究生科研创新项目(No.CX2017B557)资助

Pb2+-Induced Allosteric PS2.M as A Label-Free Colorimetric Sensor for Detection of Cumulative Radon Radiation

Corresponding author:  LYU Chang-Yin, lchy1955@163.com

Received Date:  2017-08-05

Accepted Date:  2018-03-31

Published Date:  2018-05-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 81473021) and Graduate Student Research Innovation Project in Hunan Province, China (No. CX2017B557)

以氡辐射衰变最终形成稳定的子体铅作为目标检测物,建立了检测氡累积浓度的核酸适配体生物分析新方法。K+诱导富G单链核苷酸PS2.M形成反平行G-四链体四链体,辅因子血红素与G-四联链体结合,形成具有过氧化物酶活性的稳定复合物,催化TMB-H2O2体系发生显色反应。同时加入Pb2+时,Pb2+诱导PS2.M形成结构更加稳定的"缺口"型反平行G-四链体,K+和血红素游离在溶液之中,体系吸光度响应值急剧降低。基于此构建了一种基于Pb2+诱导PS2.M构象改变的过氧化物酶活性"Turn-off"型比色传感器。在5.0×10-9~1.8×10-7 mol/L范围内,吸光度降低值ΔA与Pb2+浓度呈线性关系,线性回归方程为ΔA=0.36+0.13CR=0.9987。本方法对Pb2+的检出限为3.76 nmol/L(S/N=3),氡的检出限为1.96×103 Bq·h/m3S/N=3)。本方法灵敏、准确,样品溶液可直接检测,操作简单,避免了现场进行氡辐射剂量测量的放射性危害,为环境中氡的检测提供了新方法。

关键词:   , 子体铅, 核酸适配体, G-四链体, 构象转变, 比色传感
Key words:   Radon, Lead, Aptamer, G-quadruplex, Conformation transformation, Colorimetric sensor
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目录

铅离子诱导PS2.M构象改变特性及其氡非标记比色传感检测新方法

刘红文, 季颖, 宋春丽, 田刚, 李诗雅, 杨桂英, 吕昌银

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