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引用本文:   白雪洁, 于文静, 汪亿晗, 石磊, 苏蕊, 徐锐锋, 陈大舟, 杨洪梅, 刘淑莹. 应用电子捕获解离技术结合金属离子快速识别寡糖异构体. 分析化学, 2018, 46(1): 55-60. doi:  10.11895/j.issn.0253-3820.171261 [复制]

Citation:   BAI Xue-Jie, YU Wen-Jing, WANG Yi-Han, SHI Lei, SU Rui, XU Rui-Feng, CHEN Da-Zhou, YANG Hong-Mei, LIU Shu-Ying. Rapid Identification of Oligosaccharide Isomers by Electron Capture Dissociation in Combination with Metal Ions. Chinese Journal of Analytical Chemistry, 2018, 46(1): 55-60. doi: 10.11895/j.issn.0253-3820.171261 [复制]

应用电子捕获解离技术结合金属离子快速识别寡糖异构体

通讯作者:  陈大舟, chendz@nim.ac.cn; 杨洪梅, yanghm0327@sina.cn

收稿日期: 2017-09-20

接受日期: 2017-10-06

出版日期: 2018-01-20

基金项目: 本文系吉林省科技厅项目(Nos.20170623026TC,20160101220JC,20160204027YY)和吉林省卫生技术创新项目(No.2016J098)资助

Rapid Identification of Oligosaccharide Isomers by Electron Capture Dissociation in Combination with Metal Ions

Corresponding author:  CHEN Da-Zhou, chendz@nim.ac.cn; YANG Hong-Mei, yanghm0327@sina.cn

Received Date:  2017-09-20

Accepted Date:  2017-10-06

Published Date:  2018-01-20

Fund Project:  This work was supported by the Science and Technology Development Planning Project of Jilin Province, China (Nos. 20170623026TC, 20160101220JC, 20160204027YY) and the Health Technology Innovation Project of Jilin Province, China (No.2016J098)

应用傅里叶变换离子回旋共振质谱(FT-ICR-MS)的电子捕获解离(ECD)技术对寡糖异构体进行识别。以麦芽七糖、甘露六糖和昆布六糖为研究对象,通过在样品中添加过渡金属离子(Na+、Ca2+、Ba2+、Mg2+、Mn2+和Co2+),研究不同金属离子对寡糖碎裂的影响。加合Ba2+和Ca2+后的麦芽七糖得到了相同的碎裂离子(0,2A和2,4A),Mg2+和Mn2+也具有相似的影响(0,2A、2,4A和2,5A),Co2+加合的麦芽七糖也得到了3个交叉环断裂离子(1,4A、2,4A和2,5A),但对于加合Na+后的麦芽七糖仅得到了一个交叉环断裂离子(0,2A)。研究发现,六聚糖的质谱图的信号比相应的麦芽七糖的信号差,应是缔合的金属离子的数量不同导致的结果;区分寡糖异构体比较好的金属离子为Ca2+、Co2+和Mg2+,其中Ca2+是区分寡糖异构体的更可靠的电荷载体。

关键词:   傅里叶变换离子回旋共振质谱, 电子捕获解离, 金属离子, 寡糖异构体
Key words:   Fourier transform-ion cyclotron resonance-mass spectrometry, Electron capture dissociation, Metal ion, Oligosaccharide isomer
[1]

Hakomori S. Proc. Natl. Acad. Sci. USA, 2002, 99(1): 225-232. doi: 10.1073/pnas.012540899

[2]

Zhang X. Curr. Med. Chem., 2006, 13(10): 1141-1147. doi: 10.2174/092986706776360897

[3]

Dwek R A. Chem. Rev., 1996, 96(2): 683-720. doi: 10.1021/cr940283b

[4]

Yin H, Du Y, Zhang J. Curr. Top. Med. Chem., 2009, 9(16): 1546-1559. doi: 10.2174/156802609789909795

[5]

Bock K, Schuster-Kolbe J, Altman E, Allmaier G, Stahl B, Christian R, Sleytr U B, Messner P. J. Biol. Chem., 1994, 269(10): 7137-7144

[6]

ZaÈhringer U, Wagner F, Rietschel E T, Ben-Menachem G, Deutsch J, Rottem S. J. Biol. Chem., 1997, 272(42): 26262-26270. doi: 10.1074/jbc.272.42.26262

[7]

Harvey D J. Mass Spectrom. Rev., 2011, 30(1): 1-100. doi: 10.1002/mas.v30.1

[8]

Dell A, Morris H R. Science, 2001, 291(5512): 2351-2356. doi: 10.1126/science.1058890

[9]

Yang H, Yu Y, Song F, Liu S. J. Am. Soc. Mass Spectrom., 2011, 22(5): 845-855. doi: 10.1007/s13361-011-0085-0

[10]

Yang H, Shi L, Yao W, Huang L, Wang Y, Wan D, Liu S. J. Am. Soc. Mass Spectrom., 2015, 26(9): 1599-1605. doi: 10.1007/s13361-015-1192-0

[11]

Yang H, Shi L, Yao W, Zhuang X, Wan D, Song F, Li J, Liu S. Sci. Rep., 2016, 6: 28079-. doi: 10.1038/srep28079

[12]

Yuan H, Ying J, Deng P, Chen P, Shi J, Liu Y, Gao X, Zhao Y. Analyst, 2015, 140(23): 7965-7973. doi: 10.1039/C5AN01735A

[13]

Zubarev R A, Kelleher N L, Mclafferty F W. J. Am. Chem. Soc., 1998, 120(13): 3265-3266. doi: 10.1021/ja973478k

[14]

Zubarev R A, Kruger N A, Fridriksson E K, Lewis M A, Horn D M, Carpenter B K, Mclafferty F W. J. Am. Chem. Soc., 1999, 121(12): 2857-2862. doi: 10.1021/ja981948k

[15]

Zubarev R A, Haselmann K F, Budnik B, Kjeldsen F, Jensen F. Eur. J. Mass Spectrom., 2002, 8(5): 337-349

[16]

Syrstad E A, Stephens D D, Turecek F. J. Phys. Chem. A, 2003, 107(1): 115-126. doi: 10.1021/jp021402r

[17]

YU Jing, LI Xiao-Min, LI Hong-Mei, ZHANG Qing-He, LI Xiu-Qin, SHAO Shu-Li. Chinese J. Anal. Chem., 2015, 43(4): 564-569. doi: 10.11895/j.issn.0253-3820.140912

于晶, 李晓敏, 李红梅, 张庆和, 李秀琴, 邵淑丽. 分析化学, 2015, 43(4): 564-569. doi: 10.11895/j.issn.0253-3820.140912

[18]

Yan Y, Kusalik A J, Wu F X. IEEE-ACM Trans. Comput. Biol. Bioinform., 2017, 14(2): 337-344. doi: 10.1109/TCBB.2015.2389813

[19]

Zubarev R A, Kruger N A, Fridriksson E K, Lewis M A, Horn D M, Carpenter B K, McLafferty F W. J. Am. Chem. Soc., 1999, 121(12): 2857-2862. doi: 10.1021/ja981948k

[20]

Chen X, Chan W Y, Wong P S, Yeung H S, Chan T W. J. Am. Soc. Mass Spectrom., 2011, 22(2): 233-244. doi: 10.1007/s13361-010-0035-2

[21]

Zhang Y, Cui W, Wecksler A T, Zhang H, Molina P, Deperalta G, Gross M L. J. Am. Soc. Mass Spectrom., 2016, 27(7): 1139-1142. doi: 10.1007/s13361-016-1398-9

[22]

Adamson J T, Hakansson K. J. Proteome Res., 2006, 5(3): 493-501. doi: 10.1021/pr0504081

[23]

Budnik B A, Haselmann K F, Elkin Y N, Gorbach V I, Zubarev R A. Anal. Chem., 2003, 75(21): 5994-6001. doi: 10.1021/ac034477f

[24]

Huang Y, Pu Y, Yu X, Costello C E, Cheng L. J. Am. Soc. Mass Spectrom., 2014, 25(8): 1451-1460. doi: 10.1007/s13361-014-0921-0

[25]

Domon B, Costello C E. Glycoconj. J., 1988, 5(4): 397-409

[26]

Wan D, Jiao L, Yang H, Liu S. Planta, 2012, 235(6): 1289-1297. doi: 10.1007/s00425-011-1574-x

[27]

Cancilla M T, Penn S G, Carroll J A, Lebrilla C B. J. Am. Chem. Soc., 1996, 118(28): 6736-6745. doi: 10.1021/ja9603766

[28]

Seipert R R, Dodds E D, Clowers B H, Beecroft S M, German J B, Lebrilla C B. Anal. Chem., 2008, 80(10): 3684-3692. doi: 10.1021/ac800067y

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目录

应用电子捕获解离技术结合金属离子快速识别寡糖异构体

白雪洁, 于文静, 汪亿晗, 石磊, 苏蕊, 徐锐锋, 陈大舟, 杨洪梅, 刘淑莹

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