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引用本文:   张程, 严天旸, 陈川, 陆豪杰. 氟固相萃取辅助的生物分子质谱分析新方法. 分析化学, 2017, 45(12): 1857-1864. doi:  10.11895/j.issn.0253-3820.171297 [复制]

Citation:   ZHANG Cheng, YAN Tian-Yang, CHEN Chuan, LU Hao-Jie. Fluorous Solid-Phase Extraction Assisted New Methods for Analysis of Biomolecules with Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2017, 45(12): 1857-1864. doi: 10.11895/j.issn.0253-3820.171297 [复制]

氟固相萃取辅助的生物分子质谱分析新方法

通讯作者:  陆豪杰, luhaojie@fudan.edu.cn

收稿日期: 2017-10-07

接受日期: 2017-11-07

出版日期: 2017-12-20

基金项目: 本文系国家重点基础研究发展计划(973计划)(No.2016YFA0501303)和国家自然科学基金项目(No.21335002)资助

Fluorous Solid-Phase Extraction Assisted New Methods for Analysis of Biomolecules with Mass Spectrometry

Corresponding author:  LU Hao-Jie, luhaojie@fudan.edu.cn

Received Date:  2017-10-07

Accepted Date:  2017-11-07

Published Date:  2017-12-20

Fund Project:  This work was supported by the National Key Research and Development Program of China (No.2016YFA0501303) and the National Natural Science Foundation of China (No. 21335002)

氟固相萃取(Fluorous solid-phase extraction,FSPE)是一种基于全氟化合物之间氟-氟相互作用的固相萃取技术,通过在目标分子上进行氟标签衍生,利用高氟化固相吸附剂实现特异性的分离纯化。这一技术在有机合成、催化,以及化学和生物分离分析等诸多领域应用广泛。近年来,由于氟固相萃取和生物质谱技术之间良好的兼容性,两者联用结合的分析方法受到了研究者的广泛关注。本文在简要介绍氟固相萃取技术原理的基础之上,重点综述了其在生物质谱分析领域中的应用,并对其发展前景进行了展望。

关键词:   氟固相萃取, 生物质谱, 分离技术, 蛋白质组, 糖组, 评述
Key words:   Fluorous solid-phase extraction, Biological mass spectrometry, Separation technique, Proteomics, Glycomics, Review
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氟固相萃取辅助的生物分子质谱分析新方法

张程, 严天旸, 陈川, 陆豪杰

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