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引用本文:   秦格格, 李文慧, 徐家超, 寇晓龙, 赵容, 罗放, 方晓红. 原子力显微镜-荧光显微镜联用技术在活细胞单分子检测中的应用. 分析化学, 2017, 45(12): 1813-1823. doi:  10.11895/j.issn.0253-3820.171313 [复制]

Citation:   QIN Ge-Ge, LI Wen-Hui, XU Jia-Chao, KOU Xiao-Long, ZHAO Rong, LUO Fang, FANG Xiao-Hong. Development of Integrated Atomic Force Microscopy and Fluorescence Microscopy for Single-Molecule Analysis in Living Cells. Chinese Journal of Analytical Chemistry, 2017, 45(12): 1813-1823. doi: 10.11895/j.issn.0253-3820.171313 [复制]

原子力显微镜-荧光显微镜联用技术在活细胞单分子检测中的应用

通讯作者:  方晓红, xfang@iccas.ac.cn

收稿日期: 2017-10-09

接受日期: 2017-10-30

出版日期: 2017-12-20

基金项目: 本文系国家自然科学基金项目(Nos.21735006,91413119)资助

Development of Integrated Atomic Force Microscopy and Fluorescence Microscopy for Single-Molecule Analysis in Living Cells

Corresponding author:  FANG Xiao-Hong, xfang@iccas.ac.cn

Received Date:  2017-10-09

Accepted Date:  2017-10-30

Published Date:  2017-12-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21735006, 91413119)

原子力显微镜(Atomic force microscopy,AFM)及荧光显微镜(Fluorescence microscopy,FM)是目前活细胞单分子分析检测中最常用的两种工具。结合两种显微镜的优势,发展高时空分辨、多功能的AFM-FM联用技术成为近年该领域的研究热点。本文简述了AFM单分子力谱和FM单分子荧光成像的原理,总结了AFM-FM联用系统在仪器研制方面的发展概况,并结合本课题组在应用AFM-FM联用技术研究细胞膜上配受体相互作用等方面的工作,介绍了其在活细胞单分子检测中的应用进展。

关键词:   原子力显微镜, 荧光显微镜, 单分子荧光成像, 单分子力谱, 单分子分析, 评述
Key words:   Atomic force microscopy, Fluorescence microscopy, Single-molecule fluorescence imaging, Single-molecule force spectroscopy, Single-molecule analysis, Review
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目录

原子力显微镜-荧光显微镜联用技术在活细胞单分子检测中的应用

秦格格, 李文慧, 徐家超, 寇晓龙, 赵容, 罗放, 方晓红

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