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引用本文:   芦晋, 任雪, 刘相良, 刘泽锋. 超高效液相色谱-四极杆-静电场轨道阱质谱联用法用于宫颈癌患者尿液代谢组学研究. 分析化学, 2018, 46(1): 67-73. doi:  10.11895/j.issn.0253-3820.171412 [复制]

Citation:   LU Jin, REN Xue, LIU Xiang-Liang, LIU Ze-Feng. A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2018, 46(1): 67-73. doi: 10.11895/j.issn.0253-3820.171412 [复制]

超高效液相色谱-四极杆-静电场轨道阱质谱联用法用于宫颈癌患者尿液代谢组学研究

通讯作者:  刘泽锋, liuzefeng1129@sina.com

收稿日期: 2017-11-09

接受日期: 2017-12-05

出版日期: 2018-01-20

A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry

Corresponding author:  LIU Ze-Feng, liuzefeng1129@sina.com

Received Date:  2017-11-09

Accepted Date:  2017-12-05

Published Date:  2018-01-20

利用超高效液相色谱-四极杆-静电场轨道阱质谱联用(UHPLC-Q-Orbitrap MS)方法对宫颈癌(Cervical cancer,CC)患者和健康人(Healthy control,HC)的尿液进行分析,研究宫颈癌患者尿液中的潜在标志物,为其发病机制和诊断提供科学依据。筛选11例宫颈癌患者(Age(45.7±5.6)years)及11例健康人(Age(45.9±3.2)years)尿液样本,采用液相色谱-质谱联用技术对尿液进行测定,通过主成分分析(PCA)和偏最小二乘法-判别分析(OPLS-DA)处理数据,结果表明,两组人群代谢轮廓有显著差别,发现并鉴定了12种潜在的生物标志物,提示特定的肿瘤代谢途径中潜在的代谢标志物可能在宫颈癌发生发展中发挥重要作用。

关键词:   宫颈癌, 超高效液相色谱-四极杆-静电场轨道阱质谱, 代谢组学, 尿液, 生物标志物
Key words:   Cervical cancer, Ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry, Metabonomics, Urine, Biomarkers
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目录

超高效液相色谱-四极杆-静电场轨道阱质谱联用法用于宫颈癌患者尿液代谢组学研究

芦晋, 任雪, 刘相良, 刘泽锋

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