首页 杂志概况 投稿须知 在线投稿 在线阅读 征订启事 广告服务 行业资讯 企业动态 资料中心  专访报道 会展信息 ENGLISH

引用本文:   张何, 王青, 傅昕, 赵智粮. 基于滚环扩增及串联G-四链体-血红素DNA酶的高灵敏“Turn-on”型Hg2+传感器研究. 分析化学, 2018, 46(10): 1644-1651. doi:  10.11895/j.issn.0253-3820.181346 [复制]

Citation:   ZHANG He, WANG Qing, FU Xin, ZHAO Zhi-Liang. Fabrication of "Turn-On" Sensor for Sensitive Detection of Hg2+ Based on Rolling Circle Amplification and Tandem G-quadruplex-hemin DNAzymes. Chinese Journal of Analytical Chemistry, 2018, 46(10): 1644-1651. doi: 10.11895/j.issn.0253-3820.181346 [复制]

基于滚环扩增及串联G-四链体-血红素DNA酶的高灵敏“Turn-on”型Hg2+传感器研究

通讯作者:  张何, mzhang_he@126.com

收稿日期: 2018-05-24

接受日期: 2018-07-11

出版日期: 2018-10-20

基金项目: 本文系国家自然科学基金项目(No.21005067)和湖南省教育厅资助科研项目(Nos.17A044,16B060)资助

Fabrication of "Turn-On" Sensor for Sensitive Detection of Hg2+ Based on Rolling Circle Amplification and Tandem G-quadruplex-hemin DNAzymes

Corresponding author:  ZHANG He, mzhang_he@126.com

Received Date:  2018-05-24

Accepted Date:  2018-07-11

Published Date:  2018-10-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 21005067) and the Scientific Research Fund of Hunan Provincial Education Department (Nos. 17A044, 16B060)

以聚苯乙烯微球为载体,利用滚环放大技术,发展了一种以串联G-四链体-血红素DNA酶催化及T-Hg2+-T特异识别为基础的"Turn-on"型Hg2+高灵敏生物传感器,用于尿液样本中Hg2+的高效检测。通过链霉亲和素和生物素的特异性结合,将富T生物素化Hg2+捕获探针固定至微球表面,当Hg2+存在时,通过形成T-Hg2+-T结构将含有G-四链体互补序列的环化单链DNA序列捕获至微球表面,滚环扩增后在微球表面产生大量包含串联G-四链体的DNA序列。当氯化血红素(Hemin)插入G-四链体后,形成具有增强催化活性的G-四链体-hemin DNA酶,可催化ABTS和H2O2反应形成ABTS·+,在420 nm处具有最大吸收。考察了多种因素对检测体系的影响,在最优实验条件下,此方法对Hg2+的线性检测范围为0.4~100 pmol/L,检出限为0.3 pmol/L(S/N=3),回归方程为ΔA420 nm=0.1+0.0019CHg2+(pmol/L)。当共存离子大量存在时,传感器对Hg2+仍然具有高的选择性。应用于尿液样品中Hg2+检测,加标回收率为94.0%~106.0%,相对标准偏差(RSD)为1.4%~2.6%。此方法具有良好的选择性、灵敏度及抗干扰能力,可用于复杂样品中Hg2+的检测。

关键词:   G-四链体-血红素DNA酶, 滚环放大, 汞离子, 尿液分析, 比色分析
Key words:   G-quadruplex-hemin DNAzyme, Rolling circle amplification, Mercury ion (Ⅱ), Urine analysis, Colorimetric analysis
[1]

Chen G, Guo Z, Zeng G, Tang L. Analyst, 2015, 140: 5400-5443. doi: 10.1039/C5AN00389J

[2]

Nolan E M, Lippard S J. Chem. Rev., 2008, 108: 3443-3480. doi: 10.1021/cr068000q

[3]

Wang M, Feng W, Shi J, Zhang F, Wang B, Zhu M, Li B, Zhao Y, Chai Z. Talanta, 2007, 71(5): 2034-2039. doi: 10.1016/j.talanta.2006.09.012

[4]

Butler O T, Cook J M, Harrington C F, Hill S J, Rieuwerts J, Miles D L. J. Anal. At. Spectrum., 2006, 21: 217-243. doi: 10.1039/B516025C

[5]

Leermakers M, Baeyens W, Quevauviller P, Horvat M. TrAC-Trend. Anal. Chem., 2005, 24: 383-393. doi: 10.1016/j.trac.2004.01.001

[6]

Zheng C B, Li Y, Ma Q, Hou X D. J. Anal. At. Spectrom., 2005, 20(8): 746-750. doi: 10.1039/b503727a

[7]

Lu J Q, He X W, Zeng X S, Wan Q J, Zhang Z Z. Talanta, 2003, 59(3): 553-560. doi: 10.1016/S0039-9140(02)00569-6

[8]

JIAO Yang, ZHENG Shu-Dan, ZHOU Peng. Chin. J. Lumin., 2016, 37(3): 379-385

焦扬, 郑舒丹, 周硼. 发光学报, 2016, 37(3): 379-385

[9]

Zhou J, Bourdoncle A, Rosu F. Angew. Chem. Int. Edit., 2012, 51(44): 11002-11005. doi: 10.1002/anie.201205390

[10]

Bai D M, Ji D Y, Shang J H, Hu Y L, Gao J, Lin Z, Ge J, Li Z H. Sens. Actuators B, 2017, 252: 1146-1152. doi: 10.1016/j.snb.2017.07.181

[11]

Ji D Y, Meng H M, Ge J, Zhang L, Wang H Q, Bai D M, Li J J, Qu L B, Li Z H. Microchim. Acta, 2017, 9: 3325-3332

[12]

FU Xin, GU Dan-Yu, ZHAO Sheng-Dong, WEN Shi-Tong, ZHANG He. Chinese J. Anal. Chem., 2016, 44(10): 1487-1494. doi: 10.11895/j.issn.0253-3820.160508

傅昕, 顾丹玉, 赵圣东, 温世彤, 张何. 分析化学, 2016, 44(10): 1487-1494. doi: 10.11895/j.issn.0253-3820.160508

[13]

Guo Y, Chen J, Cheng M, Monchaud D, Zhou J, Ju H. Angew. Chem. Int. Edit., 2017, 56(52): 16636-16640. doi: 10.1002/anie.201708964

[14]

Xu M, Gao Z, Wei Q, Chen G, Tang D. Biosens. Bioelectron., 2015, 74: 1-7. doi: 10.1016/j.bios.2015.05.056

[15]

Shimron S, Wang F, Orbach R, Willner I. Anal. Chem., 2012, 84: 1042-1048. doi: 10.1021/ac202643y

[16]

KONG De-Ming. Process. Chem., 2011, 23(10): 2119-2131

孔德明. 化学进展, 2011, 23(10): 2119-2131

[17]

Wang F, Lu C H, Willner I. Chem. Rev., 2014, 114: 2881-2941. doi: 10.1021/cr400354z

[18]

Zhou W, Liang W, Li D, Yuan R, Xiang Y. Biosens. Bioelectron., 2016, 85: 573-579. doi: 10.1016/j.bios.2016.05.058

[19]

Xiao Y, Pavlov V, Niazov T, Dishon A, Kotler M, Willner I. J. Am. Chem. Soc., 2004, 126: 7430-7431. doi: 10.1021/ja031875r

[20]

Li D, Shlyahovsky B, Elbaz J, Willner I. J. Am. Chem. Soc., 2007, 129: 5804-5805. doi: 10.1021/ja070180d

[21]

Jia S M, Liu X F, Li P, Kong D M, Shen H X. Biosens. Bioelectron., 2011, 27: 148-152. doi: 10.1016/j.bios.2011.06.032

[22]

LIU Chen-Guang, WANG Jiu-Jun, ZHANG Xing-Ping, YANG Hua-Lin. Chinese J. Anal. Chem., 2017, 45(2): 163-168

刘晨光, 王久军, 张兴平, 杨华林. 分析化学, 2017, 45(2): 163-168

[23]

Zhang Q, Cai Y, Li H, Kong D M, Shen H X. Biosens. Bioelectron., 2012, 38: 331-336. doi: 10.1016/j.bios.2012.06.011

[24]

Kong D M, Wang N, Guo X X, Shen H X. Analyst, 2010, 135: 545-549. doi: 10.1039/b924014d

[25]

Ren W, Zhang Y, Huang W T, Li N B, Luo H Q. Biosens. Bioelectron., 2015, 68: 266-271. doi: 10.1016/j.bios.2015.01.010

[26]

ZHAO Qiu-Ling, LIU Ling-Ling, YANG Li-Na, ZHANG Zhen-Yu. Chinese J. Anal. Chem., 2014, 42(5): 683-688

赵秋伶, 刘玲玲, 杨丽娜, 张振宇. 分析化学, 2014, 42(5): 683-688

[27]

Li H L, Xu J G, Wang Z M, Wu Z S, Jia L. Biosens. Bioelectron., 2016, 86: 1067-1073. doi: 10.1016/j.bios.2016.07.095

[28]

Zhang P P, Wang L, Zhao H Y, Xu X W, Jiang W. Anal. Chim. Acta, 2018, 1001: 119-124. doi: 10.1016/j.aca.2017.11.036

[29]

Fernández E, Vidal L, Costa-García A, Canals A. Anal. Chim. Acta, 2016, 915: 49-55. doi: 10.1016/j.aca.2016.02.028

[30]

Fong B M, Siu T S, Lee J S, Tam S. J. Anal. Toxicol., 2007, 31: 281-287. doi: 10.1093/jat/31.5.281

[31]

ZHAO Qiu-Ling, ZHANG You-Hua, YU Xiao-Yan. Chinese J. Anal. Chem., 2016, 44(7): 1099-1105

赵秋伶, 张尤华, 于晓艳. 分析化学, 2016, 44(7): 1099-1105

[32]

She P, Chu Y, Liu C, Guo X, Zhao K, Li J, Du H, Zhang X, Wang H, Deng A. Anal. Chim. Acta, 2016, 906: 139-147. doi: 10.1016/j.aca.2015.12.021

[33]

Li F, Feng Y, Liu S F, Tang B. Chem. Commun., 2011, 47: 6347-6349. doi: 10.1039/c1cc11858g

[34]

Wang G, Xu G, Zhu Y, Zhang X. Chem. Commun., 2014, 50: 747-750. doi: 10.1039/C3CC46716C

计量
  • PDF下载量(39)
  • 文章访问量(809)
  • HTML全文浏览量(9)

目录

基于滚环扩增及串联G-四链体-血红素DNA酶的高灵敏“Turn-on”型Hg2+传感器研究

张何, 王青, 傅昕, 赵智粮

Figures and Tables