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引用本文:   李双, 洪梦蓉, 王春芳, 陈树兵, 徐继林, 陈娟娟. 13C稳定同位素示踪技术在小硅藻光合膜脂合成途径中的应用. 分析化学, 2020, 48(6): 786-793. doi:  10.19756/j.issn.0253-3820.191257 [复制]

Citation:   LI Shuang , HONG Meng-Rong , WANG Chun-Fang , CHEN Shu-Bing , XU Ji-Lin , CHEN Juan-Juan . Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on 13C Stable-Isotope-Tracer Technique. Chinese Journal of Analytical Chemistry, 2020, 48(6): 786-793. doi: 10.19756/j.issn.0253-3820.191257 [复制]

13C稳定同位素示踪技术在小硅藻光合膜脂合成途径中的应用

收稿日期: 2019-05-06

基金项目: 本文系国家重点研发计划项目(No.2018YFD0901103)、宁波大学王宽诚基金(K.C.Wong Magna)和浙江省基础公益研究计划项目(No.LGN18B050002)资助

Investigation of Synthetic Pathway of Photosynthetic Membrane Lipids in Nitzschiaclosterium f. Minutissima Based on 13C Stable-Isotope-Tracer Technique

Received Date:  2019-05-06

Fund Project:  This work was supported by the National Key R&D Program of China (No.2018YFD0901103), the K. C. Wong Magna Fund in Ningbo University, and the Projects in Zhejiang Province, China (No. LGN18B050002).

采用13C稳定同位素示踪技术结合高分辨质谱方法,研究了小硅藻中4类光合膜脂(二酰甘油单半乳糖脂(MGDG)、二酰甘油双半乳糖脂(DGDG)、二酰甘油硫代糖脂(SQDG)和磷脂酰甘油(PG))的合成途径。与通过物质含量的变化进行合成与代谢途径研究的传统方法相比,本方法更直观、准确地阐明了小硅藻光合膜脂中碳原子的去向。研究结果表明,小硅藻中4类光合膜脂均被标记,且标记含量在小硅藻生长周期的整个平台期逐渐增加。对二级质谱碎裂片段分析结果表明,光合膜脂的空间结构在13C标记方面起着决定性作用,即4类光合膜脂的极性头部被优先标记,其次是甘油骨架,最后是两条脂肪酸酰基链,且两条脂酰链的位置差别影响不大,几乎是同步合成。本方法可以直观准确地阐明小硅藻中光合膜脂对碳原子的利用途径,为相关研究提供了参考。

关键词:   13C稳定同位素示踪, 小硅藻, 光合膜脂, 四极杆/静电场轨道阱高分辨质谱
Key words:   13C Stable-isotope-tracer technique, Nitzschiaclosterium f. minutissima, Photosynthetic membrane lipids, Quadrupole electrostatic field orbital trap high resolution mass spectrometry
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13C稳定同位素示踪技术在小硅藻光合膜脂合成途径中的应用

李双, 洪梦蓉, 王春芳, 陈树兵, 徐继林, 陈娟娟

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