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引用本文:   肖坤, 龚灿, 郭强胜, 许旭. 定量核磁共振碳谱同时测定食用油中甘油三酯的sn-1,3和sn-2脂肪酸含量. 分析化学, 2020, 48(6): 802-810. doi:  10.19756/j.issn.0253-3820.191420 [复制]

Citation:   XIAO Kun , GONG Can , GUO Qiang-Sheng , XU Xu . Simultaneous Determination of Fatty Acids at sn-1,3 and sn-2 of Triglyceride in Edible Oils by Quantitative 13C-Nuclear Magnetic Resonance. Chinese Journal of Analytical Chemistry, 2020, 48(6): 802-810. doi: 10.19756/j.issn.0253-3820.191420 [复制]

定量核磁共振碳谱同时测定食用油中甘油三酯的sn-1,3和sn-2脂肪酸含量

收稿日期: 2019-07-19

基金项目: 本文系国家自然科学基金项目(No.31671928)和上海市自然科学基金项目(No.15ZR1440800)资助

Simultaneous Determination of Fatty Acids at sn-1,3 and sn-2 of Triglyceride in Edible Oils by Quantitative 13C-Nuclear Magnetic Resonance

Received Date:  2019-07-19

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 31671928) and the Natural Science Foundation of Shanghai, China (No. 15ZR1440800).

建立了定量核磁共振碳谱(13C-qNMR)技术测定食用油中特定位置不饱和脂肪酸含量的方法。使用反转门控去耦技术对食用油中的脂肪酸进行位置特异性分析,针对未完全分离的谱峰,比较了直接普通积分、使用不同的洛伦兹/高斯函数比值去卷积积分等数据处理方式对核磁共振碳谱定量结果的影响,选择以洛伦兹-高斯(3:2)的比例,对谱图进行去卷积拟合,提取sn-1,3sn-2位亚油酸和油酸两种不饱和脂肪酸的定量峰,测定3种食用油中甘油三酯的饱和脂肪酸、sn-1,3位亚油酸、sn-2位亚油酸、sn-1,3位油酸、sn-2位油酸含量分别为:大豆油(16.2%、27.8%、24.0%、15.5%、7.9%(w/w,下同));玉米油(15.3%、30.7%、20.5%、20.1%、13.3%);花生油(18.3%、18.7%、12.5%、24.9%、25.5%)。大豆油中sn-1,3位亚麻酸、sn-2位亚麻酸分别为4.5%和4.0%,玉米油和花生油中未检出亚麻酸。上述结果与1H-qNMR测定的各脂肪酸总量一致。13C-qNMR可对食用油中脂肪酸进行位置特异性分析,无需复杂的样品前处理过程,可区分不饱和脂肪酸的不同位置分布,为在缺乏甘油三酯标样的情况下测量食用油中特定甘油三酯位置异构体成分含量提供了新方法。

关键词:   定量核磁共振波谱法, 核磁共振碳谱, 食用油, 脂肪酸, 位置异构体
Key words:   Quantitative nuclear magnetic resonance, 13C-nuclear magnetic resonance, Edible oil, Fatty acid, Positional isomer
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定量核磁共振碳谱同时测定食用油中甘油三酯的sn-1,3和sn-2脂肪酸含量

肖坤, 龚灿, 郭强胜, 许旭

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