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引用本文:   屠钰, 李无双, 朗文成, 马琳, 武国华. 串联质谱法实时直接测定茶叶中γ-氨基丁酸含量. 分析化学, 2020, 48(9): 1252-1259. doi:  10.19756/j.issn.0253-3820.191665 [复制]

Citation:   TU Yu , LI Wu-Shuang , LANG Wen-Cheng , MA Lin , WU Guo-Hua . Determination of γ-Aminobutyric Acid in Different Tea Samples by Triple Quadrupole Mass Spectrometry in Real Time. Chinese Journal of Analytical Chemistry, 2020, 48(9): 1252-1259. doi: 10.19756/j.issn.0253-3820.191665 [复制]

串联质谱法实时直接测定茶叶中γ-氨基丁酸含量

通讯作者:  武国华, ghwu@just.edu.cn

收稿日期: 2019-11-11

基金项目: 本文系江苏省特聘教授专项基金项目(No.苏教师[2015]17)资助

Determination of γ-Aminobutyric Acid in Different Tea Samples by Triple Quadrupole Mass Spectrometry in Real Time

Corresponding author:  WU Guo-Hua , ghwu@just.edu.cn

Received Date:  2019-11-11

Fund Project:  This work was supported by the Jiangsu Specially Appointed Professor Program (No. Sujiaoshi([2015]17).

对茶叶中γ-氨基丁酸(γ-Aminobutyric acid,GABA)等多种功能性活性物质的快速检测,有利于茶叶资源的开发和有效利用。本研究建立了一种利用实时直接分析(DART)离子源与三重四极杆串联质谱联用快速高效定量分析茶叶中GABA含量的方法,测定了10种茶叶中GABA的含量。结果表明,不同茶叶中GABA的含量存在较大差异,其中九香翠芽中GABA含量最高,达到227.2 μg/g;其次是信阳毛尖,含量为161.2 μg/g;日照绿茶的GABA含量最低,为75.6 μg/g。本方法测得的结果与2,4-二硝基氟苯柱前衍生高效液相色谱(HPLC)检测法有较好的一致性。本方法简便、高效,为快速筛选具有高含量GABA的茶叶品种提供了一种简单有效的方法。

关键词:   茶叶, γ-氨基丁酸, 实时, 直接分析, 三重四极杆串联质谱, 高效液相色谱
Key words:   Tea, γ-Aminobutyric acid, Real time, Direct analysis, Triple quadrupole mass spectrometry, High performance liquid chromatography
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串联质谱法实时直接测定茶叶中γ-氨基丁酸含量

屠钰, 李无双, 朗文成, 马琳, 武国华

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