引用本文: 谢冰, 周帜, 章宸, 陈路路, 霍美玲, 努尔波拉提·阿依达尔汗, 刘戈宇, 阿吉艾克拜尔·艾萨, 王中华, 再帕尔·阿不力孜. 基于植物代谢组学的新疆一枝蒿产地标志物多维度研究. 分析化学, 2020, 48(6): 733-741. doi: 10.19756/j.issn.0253-3820.191710 [复制]
Citation: XIE Bing , ZHOU Zhi , ZHANG Chen , CHEN Lu-Lu , HUO Mei-Ling , NURBOLAT Aidarhan , LIU Ge-Yu , HAJI Akber Aisa , WANG Zhong-Hua , ZEPER Abliz . A Multi-dimensional Study on Biomarkers of Geographical Origins of Artemisia Rupestris L. Based on Plant Metabolomics. Chinese Journal of Analytical Chemistry, 2020, 48(6): 733-741. doi: 10.19756/j.issn.0253-3820.191710 [复制]
基于植物代谢组学的新疆一枝蒿产地标志物多维度研究
A Multi-dimensional Study on Biomarkers of Geographical Origins of Artemisia Rupestris L. Based on Plant Metabolomics
采用基于液相色谱-串联质谱（LC-MS/MS）联用技术的植物代谢组学分析方法，以采集自阿勒泰富蕴、哈密两个地区的新疆一枝蒿植物为研究对象，开展多维度代谢组分析，从人工栽培和野生品种两种角度，花、茎、枝叶3个层面，考察产地对于内源性代谢物的影响。同时，靶向分析了25种已知代表性代谢物在不同组别中的水平；结合非靶向与靶向分析，阐明不同产地该植物的代谢特征。结果表明，无论是人工栽培品种还是野生品种，不同产地样本的代谢组之间具有显著差异，在野生品种中表现更为突出。最终，从人工栽培品种筛选出29种已知结构类别的差异代谢物，包括10种黄酮类化合物、12种绿原酸类似物、7种一枝蒿酮酸衍生物；从野生品种筛选出45种已知结构类别的差异代谢物，包括17种黄酮类化合物、14种绿原酸类似物、14种一枝蒿酮酸衍生物。25种已知代表性代谢物中，8种在人工栽培品种中、11种在野生品种中表现出了产地差异性。本研究为药用植物新疆一枝蒿的产地鉴别、质量评价体系建立提供了新的研究策略和研究基础。
The plant metabolomics methods was applied to conduct multi-dimensional metabolome analysis of Artemisia rupestris L samples collected from Aletai fuyun and Hami for the first time based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. The effect of ecological origin on endogenous metabolites was investigated from two perspectives, i. e. artificial and wild varieties, and three levels, i. e. flower, stem, and leaf. Meantime, 25 known representative metabolites in different groups were analyzed, as well as the interpreting metabolic characters of this plant of different origins. According to the results, significant discrepancies were presented in both artificial and wild varieties, especially for the latter one. For artificial varieties, 29 structural category known metabolites were screened out, including 10 flavonoids, 12 chlorogenic acid analogues, and 7 rupestonic acid derivatives; for wild varieties, 45 structural category known metabolites were screened out, including 17 flavonoids, 14 chlorogenic acid analogues, and 14 rupestonic acid derivatives. In addition, among the 25 known representative metabolites, 8 in artificial varieties and 11 in wild varieties showed differentiation in ecological origin. This study provided a novel research strategy for molecular basis origin identification and quality control of medicinal plant Artemisia rupestris L.
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基于植物代谢组学的新疆一枝蒿产地标志物多维度研究
A Multi-dimensional Study on Biomarkers of Geographical Origins of Artemisia Rupestris L. Based on Plant Metabolomics
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