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引用本文:   杨丹, 周晶, 李语静, 赵锐, 冯文南, 何劲, 王哲, 杨国程. 金纳米电极对多巴胺和5-羟色胺的电化学富集和拉曼光谱检测. 分析化学, 2020, 48(6): 742-749. doi:  10.19756/j.issn.0253-3820.191722 [复制]

Citation:   YANG Dan , ZHOU Jing , LI Yu-Jing , ZHAO Rui , FENG Wen-Nan , HE Jin , WANG Zhe , YANG Guo-Cheng . Electrochemical Enrichment and Raman Spectroscopy Detection of Dopamine and 5-Hydroxytryptamine by Gold Nanoelectrode. Chinese Journal of Analytical Chemistry, 2020, 48(6): 742-749. doi: 10.19756/j.issn.0253-3820.191722 [复制]

金纳米电极对多巴胺和5-羟色胺的电化学富集和拉曼光谱检测

收稿日期: 2019-12-06

基金项目: 本文系国家自然科学基金项目(No.21673023,21773017)资助

Electrochemical Enrichment and Raman Spectroscopy Detection of Dopamine and 5-Hydroxytryptamine by Gold Nanoelectrode

Received Date:  2019-12-06

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21673023, 21773017).

对小分子神经递质多巴胺(Dopamine,DA)和5-羟色胺(5-Hydroxytryptamine,5-HT)的同时分析和检测是生命科学领域中的研究热点。单一的电化学检测手段难以有效地识别分析物,因此,多模量的同时分析尤为重要。本研究在碳纳米电极(Carbon-nanoelectrode nanopipette,CNE-NP)的基础上,通过电化学反应在其尖端沉积金,构建了一种具有电化学特性和拉曼活性的双功能金纳米电极(Gold-nanoelectrode nanopipette,GNE-NP)。经过交流介电泳(Dielectrophoresis,DEP)富集后,可用于对低浓度的DA和5-HT的检测分析。研究结果表明,此电极实现了两者的电化学响应的增强和区分。此外,引入的银纳米颗粒(AgNPs)作为拉曼增强因子和捕获分析物的纳米颗粒,同样地经过DEP富集方法,使分析物的拉曼信号增强了2个数量级,且能对共混物进行特征峰的区分。将此电极应用于血浆样品分析,可得到富集增强的电化学响应,但电化学响应会受到富集次数的限制。

关键词:   基于纳米孔道的金纳米电极, 多巴胺, 5-羟色胺, 介电泳富集, 表面增强拉曼光谱
Key words:   Gold nanoelectrode-nanopipette, Dopamine, 5-Hydroxytryptamine, Dielectrophoresis, Surface-enhanced Raman scattering
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目录

金纳米电极对多巴胺和5-羟色胺的电化学富集和拉曼光谱检测

杨丹, 周晶, 李语静, 赵锐, 冯文南, 何劲, 王哲, 杨国程

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