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引用本文:   林谆, 陈晓, 林冬果, 刘大渔. 多层纸芯片培养体系研究外泌体介导的乳腺癌细胞-肺成纤维细胞相互作用. 分析化学, 2020, 48(6): 695-702. doi:  10.19756/j.issn.0253-3820.201007 [复制]

Citation:   LIN Zhun , CHEN Xiao , LIN Dong-Guo , LIU Da-Yu . Paper-supported Co-culture System for Investigation of Exosomes Mediated Interactions between Breast Cancer Cells and Lung Fibroblasts. Chinese Journal of Analytical Chemistry, 2020, 48(6): 695-702. doi: 10.19756/j.issn.0253-3820.201007 [复制]

多层纸芯片培养体系研究外泌体介导的乳腺癌细胞-肺成纤维细胞相互作用

收稿日期: 2020-01-04

基金项目: 本文系国家自然科学基金项目(Nos.81371649,81871726,21804044)、广州市科技创新委员会科学研究项目(No.201707010277)和华南理工大学中央高校基本科研业务费专项资金项目(Nos.2018MS19,2019PY14)资助

Paper-supported Co-culture System for Investigation of Exosomes Mediated Interactions between Breast Cancer Cells and Lung Fibroblasts

Received Date:  2020-01-04

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 81371649, 81871726, 21804044), the Guangzhou Science Technology and Innovation Commission (No. 201707010277), and the Fundamental Research Funds for the Central Universities, China (Nos. 2018MS19, 2019PY14).

采用多层纸芯片共培养体系研究了外泌体介导的乳腺癌细胞-肺成纤维细胞相互作用。多层纸芯片体系包含接种乳腺癌细胞成份的肿瘤层,接种肺成纤维细胞的招募层,以及上述两层之间的侵袭层。叠加多层纸芯片形成包含乳腺癌细胞和肺成纤维细胞的三维共培养体系,而拆解多层纸芯片则逐层检测细胞,因而可以从时间和空间角度解析细胞间相互作用。研究了在肿瘤层接种乳腺癌细胞或固定乳腺癌细胞来源的外泌体,发现二者同样可以诱导招募层的肺成纤维细胞转化为肿瘤相关成纤维细胞。之后,在招募层种植外泌体诱导的肿瘤相关成纤维细胞,其显示出较原生细胞更强的乳腺癌细胞招募作用。研究结果表明,转移前微环境的形成主要在于肿瘤外泌体介导的宿主器官成纤维细胞转化,而宿主器官微环境的改变反之招募肿瘤细胞定向迁移。

关键词:   纸芯片, 乳腺癌, 成纤维细胞, 外泌体, 共培养, 转移前微环境
Key words:   Paper-based microfluidic chip, Breast cancer, Lung fibroblasts, Exosome, Co-culture, Pre-metastasis microenvironment
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多层纸芯片培养体系研究外泌体介导的乳腺癌细胞-肺成纤维细胞相互作用

林谆, 陈晓, 林冬果, 刘大渔

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