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引用本文:   张岚, 李小琴, 王光丽. 石墨相氮化碳光诱导活化辣根过氧化物酶及其比色免疫分析应用. 分析化学, 2020, 48(6): 703-711. doi:  10.19756/j.issn.0253-3820.201065 [复制]

Citation:   ZHANG Lan , LI Xiao-Qin , WANG Guang-Li . Photoswitching Enzymatic Activity of Horseradish Peroxidase by Graphite Phase Carbonitride for Colorimetric Immunoassay. Chinese Journal of Analytical Chemistry, 2020, 48(6): 703-711. doi: 10.19756/j.issn.0253-3820.201065 [复制]

石墨相氮化碳光诱导活化辣根过氧化物酶及其比色免疫分析应用

收稿日期: 2020-02-10

基金项目: 本文系国家自然科学基金项目(Nos.21575052,21676123)资助

Photoswitching Enzymatic Activity of Horseradish Peroxidase by Graphite Phase Carbonitride for Colorimetric Immunoassay

Received Date:  2020-02-10

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21575052, 21676123).

基于石墨相氮化碳(g-C3N4)在可见光(λ≥400 nm)照射下可激活辣根过氧化物酶(HRP),使HRP能够催化氧化特征底物3,3',5,5'-四甲基联苯胺(TMB),设计了一种新型夹心型免疫传感器,用于高灵敏检测甲胎蛋白(AFP)。此夹心型免疫传感器利用附着于微孔板上的一抗(Ab1)捕获目标物AFP,然后与HRP和二抗(Ab2)共同标记的金纳米颗粒(Ab2-AuNPs-HRP)反应,并结合酰胺放大技术(TSA),增大表面固定的HRP的量,实现信号放大。加入g-C3N4后,在可见光照射下,HRP的活性被激发,TMB被氧化后的信号(即652 nm处的吸光度)与AFP的浓度在0.5 pg/mL~0.1 μg/mL范围内呈线性关系,检出限(LOD,S/N=3)为0.17 pg/mL。 本研究构建的新型免疫传感器具有灵敏度高、选择性好、使用方便等优点,在实际样品测定中具有潜在的应用价值。

关键词:   石墨相氮化碳, 辣根过氧化物酶, 活化, 免疫分析
Key words:   Graphite-phase carbon nitride, Horseradish peroxidase, Activation, Immunoassay
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石墨相氮化碳光诱导活化辣根过氧化物酶及其比色免疫分析应用

张岚, 李小琴, 王光丽

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