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引用本文:   胡献丽, 朱琳嶺, 李苓菱, 徐志爱, 张文. 阳离子化荧光金纳米簇的合成及siRNA递送研究. 分析化学, 2020, 48(6): 712-720. doi:  10.19756/j.issn.0253-3820.201088 [复制]

Citation:   HU Xian-Li , ZHU Lin-Ling , LI Ling-Ling , XU Zhi-Ai , ZHANG Wen . Cationic Fluorescent Gold Nanoclusters for Nonviral siRNA Delivery. Chinese Journal of Analytical Chemistry, 2020, 48(6): 712-720. doi: 10.19756/j.issn.0253-3820.201088 [复制]

阳离子化荧光金纳米簇的合成及siRNA递送研究

收稿日期: 2020-02-19

基金项目: 本文系国家自然科学基金项目(Nos.21775046,21675055)资助

Cationic Fluorescent Gold Nanoclusters for Nonviral siRNA Delivery

Received Date:  2020-02-19

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21775046, 21675055).

基于Small interfering RNA(siRNA)的RNA干扰(RNA interference,RNAi)策略已经成为生物医学研究的常规手段,而通常采用的基于聚阳离子或阳离子磷脂的siRNA递送系统递送效率低、细胞毒性高,而且缺少示踪功能,严重制约了siRNA药物临床转化。为克服传统siRNA递送载体的不足,本研究发展了一种新型siRNA荧光纳米递送系统。首先利用牛血清白蛋白为模板,通过生物矿化策略合成具有近红外荧光发射的金纳米簇(Gold nanocluster,GN),利用乙二胺(Ethylenediamine,EDA)或N,N-二甲基乙二胺(N,N'-Dimethylethylenediamine,DMA)对GN进行共价修饰,得到阳离子化金纳米簇(Cationic gold nanocluster,CGN),同时实现siRNA高效递送和荧光示踪。然后,以表达绿色荧光蛋白(Green fluorescence protein,GFP)的肺癌A549细胞(A549-GFP)为细胞模型,验证了CGN可实现siRNA高效递送,并沉默A549-GFP细胞的GFP表达;进一步利用共聚焦激光扫描显微镜实现了GN细胞分布的荧光示踪。本研究结果表明,CGN有望用作siRNA递送载体和示踪探针。

关键词:   siRNA, 金纳米簇, 阳离子化, 荧光示踪, 递送载体
Key words:   Small interfering RNA delivery, Gold nanocluster, Cationic, Fluorescence tracing, Nanovector
[1]

Elbashir S M, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Nature,2001,411(6836):494-498

[2]

Mello C C, Conte D. Nature,2004,431(7006):338-342

[3]

de Fougerolles A, Vornlocher H P, Maraganore J, Lieberman J. Nat. Rev. Drug Discovery,2007,6(6):443-453

[4]

Resnier P, Montier T, Mathieu V, Benoit J P, Passirani C. Biomaterials, 2013,34(27):6429-6443

[5]

Shen X L, Corey D R. Nucleic Acids Res.,2018,46(4):1584-1600

[6]

Zhang X P, Goel V, Robbie G J. J. Clin. Pharmacol.,2019,DOI:10.1002/jcph.1553

[7]

Cooper B M, Putnam D. ACS Biomater. Sci. Eng.,2016,2(11):1837-1850

[8]

Lächelt U, Wagner E. Chem. Rev., 2015,115(19):11043-11078

[9]

Blagbrough I S, Metwally A A, Ghonaim H M. Mol. Pharmaceut.,2012,9(7):1853-1861

[10]

Liu L, Zheng M Y, Librizzi D, Renette T, Merkel O M, Kissel T. Mol. Pharmaceut.,2016,13(1):134-143

[11]

Patil M L, Zhang M, Minko T. ACS Nano,2011,5(3):1877-1887

[12]

Nuhn L, Hirsch M, Krieg B, Koynov K, Fischer K, Schmidt M, Helm M, Zentel R. ACS Nano,2012,6(3):2198-2214

[13]

Mudd S R, Trubetskoy V S, Blokhin A V, Weichert J P, Wolff J A. Bioconjugate Chem.,2010,21(7):1183-1189

[14]

Wu C F, Bull B, Szymanski C, Christensen K, McNeill J. ACS Nano,2008,2(11):2415-2423

[15]

Nie H L, Jing J, Tian Y, Yang W, Zhang R B, Zhang X L. ACS Appl. Mater. Interfaces,2016,8(14):8991-8997

[16]

Kobayashi H, Ogawa M, Alford R, Choyke P L, Urano Y. Chem. Rev.,2010,110(5):2620-2640

[17]

Qi L F, Gao X H. ACS Nano,2008,2(7):1403-1410

[18]

Derfus A M, Chen A A, Min D H, Ruoslahti E, Bhatia S N. Bioconjugate Chem.,2007,18(5):1391-1396

[19]

Lee H, Kim I K, Park T G. Bioconjugate Chem.,2010,21(2):289-295

[20]

Maity S, Bain D, Patra A. J. Phys. Chem. C, 2019,123(4):2506-2515

[21]

Jin R C. Nanoscale,2010,2(3):343-362

[22]

Zheng J, Nicovich P R, Dickson R M. Annu. Rev. Phys. Chem.,2007,58(1):409-431

[23]

Diez I, Ras R H. Nanoscale,2011,3(5):1963-1970

[24]

Wang Y L, Xu C, Zhai J, Gao F P, Liu R, Gao L, Zhao Y L, Chai Z F, Gao X Y. Anal. Chem.,2015,87(1):343-345

[25]

Wang Y L, Cui Y Y, Zhao Y, Liu R, Sun Z P, Li W, Gao X Y. Chem. Commun.,2012,48(6):871-873

[26]

An D Y, Su J G, Weber J K, Gao X Y, Zhou R H, Li J Y. J. Am. Chem. Soc.,2015,137(26):8412-8418

[27]

Xie J P, Zheng Y G, Ying J Y. J. Am. Chem. Soc.,2009,131(3):888-889

[28]

Wei H, Wang Z D, Yang L M, Tian S L, Hou C J, Lu Y. Analyst, 2010,135(6):1406-1410

[29]

Liu C L, Wu H T, Hsiao Y H, Lai C W, Shih C W, Peng Y K, Tang K C, Chang H W, Chien Y C, Hsiao J K, Cheng J T, Chou P T. Angew. Chem. Int. Ed.,2011,50(31):7056-7060

[30]

Thakur N S, Mandal N, Banerjee U C. ACS Omega,2018,3(12):18553-18562

[31]

Li Z, Peng H, Liu J, Tian Y, Yang W, Yao J, Shao Z, Chen X. ACS Appl. Mater. Interfaces, 2018,10(1):83-90

[32]

Wang Y, Chen J, Irudayaraj J. ACS Nano,2011,5(12):9718-9725

[33]

Lei Y F, Tang L X, Xie Y Z, Xianyu L X, Zhang L M, Wang P, Hamada Y, Jiang K, Zheng W F, Jiang X Y. Nat. Commun.,2017,8:15130

[34]

Nel A E, Madler L, Velegol D, Xia T, Hoek E M V, Somasundaran P, Klaessig F, Castranova V, Thompson M. Nat. Mater.,2009,8(7):543-557

[35]

Remaut K, Sanders N N, Geest B G D, Braeckmans K, Demeester J, Smedt S C D. Mat. Sci. Eng.,2007,58(3-5):117-161

[36]

Behr J P. CHIMIA,1997,51(1-2):34-36

[37]

Varkouhi A K, Scholte M, Storm G, Haisma H J. J. Control. Release,2011,151(3):220-228

[38]

Li N, Yang Y, Ding M, Huang W D, Li H G, Ye J, Xiao J, Zha X L, Xu H N. Mol. Biotechnol.,2014,56(12):1079-1088

[39]

Shang L, Dong S J, Nienhaus G U. Nano Today,2011,6(4):401-418

[40]

Lu Y Z, Chen W. Chem. Soc. Rev.,2012,41(9):3594-3623

[41]

Dichiarante V, Tirotta I, Catalano L, Terraneo G, Raffaini G, Chierotti M R, Gobetto R, Bombelli F B, Metrangolo P. Chem. Commun., 2017,53(3):621-624

[42]

Dominska M, Dykxhoorn D M. J. Cell Sci.,2010,123(8):1183-1189

[43]

Wittrup A, Ai A, Liu X, Hamar P, Trifonova R, Charisse K, Manoharan M, Kirchhausen T, Lieberman J. Nat. Biotechnol.,2015,33(8):870-876

[44]

Yu H J, Guo C Y, Chen X, Feng B, Liu J P, Chen X Z, Wang D G, Teng L S, Li Y X, Yin Q, Zhang Z W, Li Y P. Theranostics,2016,6(1):14-27

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阳离子化荧光金纳米簇的合成及siRNA递送研究

胡献丽, 朱琳嶺, 李苓菱, 徐志爱, 张文

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