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引用本文:   付彦丰, 杨钰, 张清荣, 蔡明军, 徐海娇, 杨国程, 单玉萍. 原子力显微镜纳米压痕技术用于评估纳米药物功效. 分析化学, 2020, 48(9): 1219-1227. doi:  10.19756/j.issn.0253-3820.201171 [复制]

Citation:   FU Yan-Feng , YANG Yu , ZHANG Qing-Rong , CAI Ming-Jun , XU Hai-Jiao , YANG Guo-Cheng , SHAN Yu-Ping . Atomic Force Microscopy-based Nanoindentation Technique for Evaluation of Efficacy of Nano-drugs. Chinese Journal of Analytical Chemistry, 2020, 48(9): 1219-1227. doi: 10.19756/j.issn.0253-3820.201171 [复制]

原子力显微镜纳米压痕技术用于评估纳米药物功效

通讯作者:  单玉萍, shanyp@mail.ccut.edu.cn

收稿日期: 2020-03-31

基金项目: 本文系国家自然科学基金项目(Nos.21773017,21673023)资助

Atomic Force Microscopy-based Nanoindentation Technique for Evaluation of Efficacy of Nano-drugs

Corresponding author:  SHAN Yu-Ping , shanyp@mail.ccut.edu.cn

Received Date:  2020-03-31

Fund Project:  This work was supported by the National Natural Science Foundation of China(Nos. 21773017, 21673023).

具有靶向性药物递送和可控释放双重优势的纳米药物是抗肿瘤药物的研究热点。在纳米药物应用中,药效评估是非常重要的方面。本研究利用基于原子力显微镜(AFM)的纳米压痕技术,通过分析纳米药物作用后细胞力学性质(杨氏模量和粘弹性等)的改变,评估其药效。结果表明,与游离的阿霉素(DOX)相比,DOX及喜树碱(CPT)脂质体纳米药物对宫颈癌(HeLa)细胞的药效作用更明显。进一步检测DOX和CPT不同摩尔比(4:1和1:4)混合的脂质体纳米药物对HeLa细胞的药效差异,发现在DOX和CPT的摩尔比为4:1条件下,两种药物的协同作用增强了药效。对纳米药物作用后的HeLa细胞进行粘弹性评估,发现随着纳米药物作用时间的延长,细胞粘弹性随之降低。本研究提供了一种简单有效的筛选纳米药物的方法。

关键词:   原子力显微镜, 纳米压痕, 脂质体, 纳米药物功效, 细胞力学
Key words:   Atomic force microscopy, Nanoindentation, Liposomes, Nano-drug efficacy, Cell mechanics
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目录

原子力显微镜纳米压痕技术用于评估纳米药物功效

付彦丰, 杨钰, 张清荣, 蔡明军, 徐海娇, 杨国程, 单玉萍

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