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引用本文:   王存, 刘蕃鑫, 惠俊敏, 邹晓川, 邓明川, 郑天平, 刘林. 基于生物金属有机骨架封装亚甲基蓝复合材料和信号放大策略高灵敏检测microRNA-141. 分析化学, 2020, 48(9): 1185-1192. doi:  10.19756/j.issn.0253-3820.201222 [复制]

Citation:   WANG Cun , LIU Fan-Xin , HUI Jun-Min , ZOU Xiao-Chuan , DENG Ming-Chuan , ZHENG Tian-Ping , LIU Lin . Biosensor Based on Methylene Blue Encapsulated Metal Organic Framework and Signal Amplification Strategy for Sensitive Detection of MicroRNA-141. Chinese Journal of Analytical Chemistry, 2020, 48(9): 1185-1192. doi: 10.19756/j.issn.0253-3820.201222 [复制]

基于生物金属有机骨架封装亚甲基蓝复合材料和信号放大策略高灵敏检测microRNA-141

通讯作者:  王存, wangcun5224@126.com

收稿日期: 2020-04-22

基金项目: 本文系重庆市教委科技研究项目(No.KJQN201901604)和重庆市自然科学基金项目(No.cstc2018jcyjAX0110)资助

Biosensor Based on Methylene Blue Encapsulated Metal Organic Framework and Signal Amplification Strategy for Sensitive Detection of MicroRNA-141

Corresponding author:  WANG Cun , wangcun5224@126.com

Received Date:  2020-04-22

Fund Project:  This work was supported by the Science and Technology Research Project of Chongqing Municipal Education Commission (No. KJQN201901604) and the Natural Science Foundation of Chongqing, China (No. cstc2018jcyjAX0110).

MicroRNA-141(miRNA-141)的检测对相关癌症的早期诊断和预后评估具有重要意义。本研究建立了一种无酶的生物传感器,用于高灵敏检测miRNA-141。首先,合成了具有多孔结构和良好生物相容性的生物金属有机骨架(bio-MOF),通过离子交换的方式,bio-MOF可以封装大量亚甲基蓝(MB)信号分子,并保持其生物活性。其次,利用目标物循环信号放大策略中的催化发夹自组装(CHA)、辅助目标miRNA-141循环,实现信号放大,进一步提高了传感器的灵敏度。在1.0×10-16-1.0×10-6 mol/L范围内,目标miRNA-141浓度的对数与传感器电化学信号呈线性相关,检出限为3.3×10-17 mol/L(S/N=3)。将此方法应用于健康人血清中miRNA-141检测,加标回收率为99.9%-102.0%(RSD≤2.1%)。此生物传感器简单、可靠、灵敏度高、线性范围宽,为高灵敏检测其它miRNA提供了参考。

关键词:   生物传感器, 催化发夹自组装, 信号放大策略, MicroRNA-141, 生物金属有机骨架, 亚甲基蓝
Key words:   Biosensor, Catalyzed hairpin assembly, Signal amplification strategy, MicroRNA-141, Bio-metal organic framework, Methylene blue
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目录

基于生物金属有机骨架封装亚甲基蓝复合材料和信号放大策略高灵敏检测microRNA-141

王存, 刘蕃鑫, 惠俊敏, 邹晓川, 邓明川, 郑天平, 刘林

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