首页 杂志概况 投稿须知 在线投稿 在线阅读 征订启事 广告服务 行业资讯 企业动态 资料中心  专访报道 会展信息 ENGLISH

引用本文:   盛楠, 马雪萍, 逄淑云, 宋沁馨, 邹秉杰, 周国华. 新型冠状病毒SARS-CoV-2核酸检测技术平台的研究进展. 分析化学, 2020, 48(10): 1279-1287. doi:  10.19756/j.issn.0253-3820.201259 [复制]

Citation:   SHENG Nan , MA Xue-Ping , PANG Shu-Yun , SONG Qin-Xin , ZOU Bing-Jie , ZHOU Guo-Hua . Research Progress of Nucleic Acid Detection Technology Platforms for New Coronavirus SARS-CoV-2. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1279-1287. doi: 10.19756/j.issn.0253-3820.201259 [复制]

新型冠状病毒SARS-CoV-2核酸检测技术平台的研究进展

通讯作者:  邹秉杰, zbj523@163.com; 周国华, ghzhou@nju.edu.cn

收稿日期: 2020-05-06

基金项目: 本文系国家自然科学基金项目(Nos.61871403,81703474,81673390)、江苏省青年医学人才项目(No.QNRC2016889)和江苏省自然科学基金项目(No.BK20180292)资助

Research Progress of Nucleic Acid Detection Technology Platforms for New Coronavirus SARS-CoV-2

Corresponding author:  ZOU Bing-Jie , zbj523@163.com; ZHOU Guo-Hua , ghzhou@nju.edu.cn

Received Date:  2020-05-06

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 61871403, 81703474, 81673390), the Jiangsu Provincial Medical Youth Talent Program (No. QNRC2016889) and the Natural Science Foundation of Jiangsu Province, China (No. BK20180292).

新型冠状病毒(SARS-CoV-2)已蔓延至全球,严重威胁人类的健康。SARS-CoV-2传染性强、潜伏期长,且存在无症状感染者,因此,准确检测SARS-CoV-2对疫情防控至关重要。核酸检测技术在防控工作中发挥了举足轻重的作用,目前已开发出多种用于SARS-CoV-2核酸检测的方法,有些方法已被开发成试剂盒应用于临床检测。然而,这些方法原理各异,检测平台也不同,因此,选择合适的SARS-CoV-2核酸检测方法对疫情防控至关重要。本文介绍了目前核酸检测方法的原理与技术平台,比较了各方法的优缺点,明确了各类方法的应用范围,以期为选择合适的SARS-CoV-2核酸检测方法提供参考,对今后开发类似SARS-CoV-2的病原体检测方法提出展望。

关键词:   新型冠状病毒, 核酸检测, 技术原理, 评述
Key words:   SARS-CoV-2, Nucleic acid detection, Technical principle, Review
[1]

Chan J F, Yuan S, Kok K H, To K K, Chu H, Yang J, Xing F, Liu J, Yip C C, Poon R W, Tsoi H W, Lo S K, Chan K H, Poon V K, Chan W M, Ip J D, Cai J P, Cheng V C, Chen H, Hui C K, Yuen K Y. Lancet, 2020, 395(10223):514-523

[2]

Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao G F, Tan W. N. Engl. J. Med., 2020, 382(8):727-733

[3]

Wang C, Horby P W, Hayden F G, Gao G F. Lancet, 2020, 395(10223):470-473

[4]

WHO. 2020-05-05. https://www.who.int/emergencies/diseases/novel-coronavirus-2019

[5]

Zhou P, Yang X L, Wang X G, Hu B, Zhang L, Zhang W, Si H R, Zhu Y, Li B, Huang C L, Chen H D, Chen J, Luo Y, Guo H, Jiang R D, Liu M Q, Chen Y, Shen X R, Wang X, Zheng X S, Zhao K, Chen Q J, Deng F, Liu L L, Yan B, Zhan F X, Wang Y Y, Xiao G F, Shi Z L. Nature, 2020, 579(7798):270-273

[6]

Jiang S, Shi Z, Shu Y, Song J, Gao G F, Tan W, Guo D. Lancet, 2020, 395(10228):949

[7]

Zhou F, Yu T, Du R, Fan G, Liu Y, Liu Z, Xiang J, Wang Y, Song B, Gu X, Guan L, Wei Y, Li H, Wu X, Xu J, Tu S, Zhang Y, Chen H, Cao B. Lancet, 2020, 395(10229):1054-1062

[8]

Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, Zhang L, Fan G, Xu J, Gu X, Cheng Z, Yu T, Xia J, Wei Y, Wu W, Xie X, Yin W, Li H, Liu M, Xiao Y, Gao H, Guo L, Xie J, Wang G, Jiang R, Gao Z, Jin Q, Wang J, Cao B. Lancet, 2020, 395(10223):497-506

[9]

Wrapp D, Wang N, Corbett K S, Goldsmith J A, Hsieh C L, Abiona O, Graham B S, McLellan J S. Science, 2020, 367(6483):1260-1263

[10]

Koo J R, Cook A R, Park M, Sun Y, Sun H, Lim J T, Tam C, Dickens B L. Lancet Infect. Dis., 2020, 20(6):678-688

[11]

Pagenkopp Lohan K M, Fleischer R C, Carney K J, Holzer K K, Ruiz G M. Microb. Ecol., 2016, 71(3):530-542

[12]

Zhang J, He S, Li Y, Lv M, Wei H, Qu B, Zheng Y, Hu C. Exp. Ther. Med., 2018, 16(5):4207-4212

[13]

Yan B, Boitano M, Clark T A, Ettwiller L. Nat. Commun., 2018, 9(1):3676

[14]

Clark M B, Wrzesinski T, Garcia A B, Hall N A L, Kleinman J E, Hyde T, Weinberger D R, Harrison P J, Haerty W, Tunbridge E M. Mol. Psychiatry, 2020, 25(1):37-47

[15]

Stewart B J, Ferdinand J R, Clatworthy M R. Nat. Rev. Nephrol., 2020, 16(2):112-128

[16]

Fritz A, Hofmann P, Majda S, Dahms E, Droge J, Fiedler J, Lesker T R, Belmann P, DeMaere M Z, Darling A E, Sczyrba A, Bremges A, McHardy A C. Microbiome, 2019, 7(1):17

[17]

Deng X, Achari A, Federman S, Yu G, Somasekar S, Bartolo I, Yagi S, Mbala-Kingebeni P, Kapetshi J, Ahuka-Mundeke S, Muyembe-Tamfum J J, Ahmed A A, Ganesh V, Tamhankar M, Patterson J L, Ndembi N, Mbanya D, Kaptue L, McArthur C, Munoz-Medina J E, Gonzalez-Bonilla C R, Lopez S, Arias C F, Arevalo S, Miller S, Stone M, Busch M, Hsieh K, Messenger S, Wadford D A, Rodgers M, Cloherty G, Faria N R, Theze J, Pybus O G, Neto Z, Morais J, Taveira N, R. Hackett J J, Chiu C Y. Nat. Microbiol., 2020, 5(3):443-454

[18]

Wu F, Zhao S, Yu B, Chen Y M, Wang W, Song Z G, Hu Y, Tao Z W, Tian J H, Pei Y Y, Yuan M L, Zhang Y L, Dai F H, Liu Y, Wang Q M, Zheng J J, Xu L, Holmes E C, Zhang Y Z. Nature, 2020, 579(7798):265-269

[19]

Wang M, Fu A, Hu B, Tong Y, Liu R, Liu Z, Gu J, Xiang B, Liu J, Jiang W, Shen G, Zhao W, Men D, Deng Z, Yu L, Wei W, Li Y, Liu T. Small, 2020, 16(32):e2002169

[20]

Schurr F, Tison A, Militano L, Cheviron N, Sircoulomb F, Riviere M P, Ribiere-Chabert M, Thiery R, Dubois E. J. Virol. Methods, 2019, 270:70-78

[21]

Pinheiro-de-Oliveira T F, Fonseca-Junior A A, Camargos M F, Laguardia-Nascimento M, Giannattasio-Ferraz S, Cottorello A C P, de Oliveira A M, Goes-Neto A, Barbosa-Stancioli E F. Transbound. Emerg. Dis., 2019, 66(3):1360-1369

[22]

Yu F, Yan L, Wang N, Yang S, Wang L, Tang Y, Gao G, Wang S, Ma C, Xie R, Wang F, Tan C, Zhu L, Guo Y, Zhang F. Clin. Infect. Dis., 2020, 71(5):793-798

[23]

Kajiwara H, Murakami R. Anal. Biochem., 2017, 539:45-47

[24]

Kim S, Park J, Na J, Jung G Y, Hwang J. PLoS One, 2016, 11(7):e0153201

[25]

Liu L, Yang D, Liu G. Biosens. Bioelectron., 2019, 136:60-75

[26]

Huang L, Tian S, Zhao W, Liu K, Ma X, Guo J. Analyst, 2020, 145(8):2828-2840

[27]

Choi J R, Hu J, Gong Y, Feng S, Wan Abas W A, Pingguan-Murphy B, Xu F. Analyst, 2016, 141(10):2930-2939

[28]

Tsaloglou M N, Nemiroski A, Camci-Unal G, Christodouleas D C, Murray L P, Connelly J T, Whitesides G M. Anal. Biochem.,2018, 543:116-121

[29]

Gootenberg J S, Abudayyeh O O, Lee J W, Essletzbichler P, Dy A J, Joung J, Verdine V, Donghia N, Daringer N M, Freije C A, Myhrvold C, Bhattacharyya R P, Livny J, Regev A, Koonin E V, Hung D T, Sabeti P C, Collins J J, Zhang F. Science, 2017, 356(6336):438-442

[30]

Zhang F, Abudayyeh O O, Gootenberg S S. (2020-03-21)2020-05-05. https://broad.io/sherlockprotocol

[31]

Broughton J P, Deng X, Yu G, Fasching C L, Servellita V, Singh J, Miao X, Streithorst J A, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C Y, Guevara H, Wadford D A, Chen J S, Chiu C Y. Nat. Biotechnol., 2020, 38(7):870-874

[32]

Jiang Q, Wu J, Yao K, Yin Y, Gong M M, Yang C, Lin F. ACS Sens., 2019, 4(11):3072-3079

[33]

Reboud J, Xu G, Garrett A, Adriko M, Yang Z, Tukahebwa E M, Rowell C, Cooper J M. Proc. Natl. Acad. Sci. USA, 2019, 116(11):4834-4842

[34]

Tang R, Yang H, Gong Y, You M, Liu Z, Choi J R, Wen T, Qu Z, Mei Q, Xu F. Lab Chip, 2017, 17(7):1270-1279

[35]

Nasseri B, Soleimani N, Rabiee N, Kalbasi A, Karimi M, Hamblin M R. Biosens. Bioelectron., 2018, 117:112-128

[36]

Bwana P, Ageng'o J, Mwau M. PLoS One, 2019, 14(3):e0213865

[37]

Qing L, Song Q X, Feng J L, Li H Y, Liu G, Jiang H H. Ann. Clin. Microbiol. Antimicrob., 2017, 16(1):45

计量
  • PDF下载量(71)
  • 文章访问量(223)
  • HTML全文浏览量(24)

目录

新型冠状病毒SARS-CoV-2核酸检测技术平台的研究进展

盛楠, 马雪萍, 逄淑云, 宋沁馨, 邹秉杰, 周国华

Figures and Tables