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引用本文:   王姜, 黄丽娟, 徐贞俊, 刘昱, 徐静娟, 陈洪渊. 膜萃取电喷雾电离质谱快速分析菌血血样的研究. 分析化学, 2020, 48(10): 1315-1324. doi:  10.19756/j.issn.0253-3820.201263 [复制]

Citation:   WANG Jiang , HUANG Li-Juan , XU Zhen-Jun , LIU Yu , XU Jing-Juan , CHEN Hong-Yuan . Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1315-1324. doi: 10.19756/j.issn.0253-3820.201263 [复制]

膜萃取电喷雾电离质谱快速分析菌血血样的研究

通讯作者:  徐静娟, xujj@nju.edu.cn

收稿日期: 2020-05-08

基金项目: 本文系国家自然科学基金项目(Nos.21327902,21974060)资助

Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry

Corresponding author:  XU Jing-Juan , xujj@nju.edu.cn

Received Date:  2020-05-08

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos.21327902, 21974060).

采用自行研制的膜萃取电喷雾电离质谱(MEESI-MS),将大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌和铜绿假单胞菌4种细菌通过血液培养后,正、负离子模式下进行质谱检测,获得其化学指纹图谱,通过主成分分析(PCA)方法对所获指纹谱图信息进行分析,并对样品进行分类。结果表明,MEESI-MS可快速获取菌血血样化学指纹谱图,结合PCA方法对菌血血样中细菌种类进行快速鉴别,菌血样品培养时间约60 min,整体分析时间少于65 min。本方法样品预处理简单、操作简便、分析速度快,可望用于临床菌血症和败血症的细菌种类的快速鉴别。

关键词:   膜萃取电喷雾电离, 质谱, 主成分分析, 菌血症, 血流感染
Key words:   Membrane extraction electrospray ionization, Mass spectrometry, Principal component analysis, Bacteremia, Bloodstream infection
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膜萃取电喷雾电离质谱快速分析菌血血样的研究

王姜, 黄丽娟, 徐贞俊, 刘昱, 徐静娟, 陈洪渊

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