基于麦氏酸活化呋喃的鱼肉新鲜度指示卡研究

引用本文: 李宁, 肖婷, 章骞, 陈晓梅, 陈曦. 基于麦氏酸活化呋喃的鱼肉新鲜度指示卡研究. 分析化学, 2020, 48(12): 1642-1649. doi: 10.19756/j.issn.0253-3820.201297 [复制]

Citation: LI Ning , XIAO Ting , ZHANG Qian , CHEN Xiao-Mei , CHEN Xi . A Color Indicator for Fish Freshness Based on Meldrum's Activated Furan. Chinese Journal of Analytical Chemistry, 2020, 48(12): 1642-1649. doi: 10.19756/j.issn.0253-3820.201297 [复制]

基于麦氏酸活化呋喃的鱼肉新鲜度指示卡研究

李宁^1,, 肖婷^1,, 章骞^1,, 陈晓梅^1,, 陈曦^2,

¹ 集美大学食品与生物工程学院, 厦门 361021;

² 厦门大学化学化工学院, 厦门 361005

收稿日期: 2020-05-24

基金项目: 本文系国家自然科学基金项目（No.21675062）和福建省特支"双百计划"资助

A Color Indicator for Fish Freshness Based on Meldrum's Activated Furan

LI Ning ^1,, XIAO Ting ^1,, ZHANG Qian ^1,, CHEN Xiao-Mei ^1,, CHEN Xi ^2,

¹ College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;

² College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 2020-05-24

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21675062) and the Top Young Innovative Talents Project in Fujian Province, China.

传统的肉类新鲜度指示卡采用pH型指示剂，受环境因素影响大，容易造成假阳性结果。本研究以麦氏酸活化呋喃（Meldrum's activated furan，MAF）为挥发性胺指示剂，聚氯乙烯（Polyvinyl chloride，PVC）为载体，设计了一种用于检测鱼肉新鲜度的MAF/PVC薄膜。通过旋转涂布法制备薄膜，以NH₃为模型物，考察了薄膜的传感性能。结果表明，MAF质量含量为15%、旋转涂布速度为2000 r/min时制得的薄膜对NH₃传感效果最好。以MAF/PVC薄膜为新鲜度指示卡，贴于装有鲜鱼肉的托盘包装内。测定MAF/PVC薄膜的亮度（L）、红色度（a）、黄色度（b）值，并根据MAF/PVC薄膜响应前后的L、a、b值计算色差值（ΔE）；同时，测定鱼肉样品中的总挥发性盐基氮（Total volatile basic nitrogen，TVB-N）含量。结果表明，MAF/PVC薄膜的ΔE值变化趋势与样品中TVB-N含量具有良好的相关性，可准确、快速指示鱼肉的新鲜度，在水产品新鲜度检测中具有良好的应用前景。

关键词: 新鲜度指示卡, 鱼肉, 麦氏酸活化呋喃

pH-sensitive material is commonly used as traditional freshness indicators, which is greatly suffered by environmental factors and easy to cause false positive results. To solve this problem, a freshness indicator using Meldrum's activated furan (MAF) as volatile amine-sensitive material and polyvinyl chloride (PVC) as supporting film was proposed in this work. MAF/PVC films were prepared by spin coating method, and the sensing properties of the films were investigated with NH₃ as the model target. Experimental results showed that the film prepared with 15% mass content of MAF at 2000 r/min of coating speed revealed the best sensing property towards NH₃. Using MAF/PVC film as the freshness indicator for fish, the lightness (L), redness (a) and yellowness (b) values of films were recorded. The color difference value (ΔE) was calculated according to the L, a, b values of the MAF/PVC films. At the same time, the TVB-N contents of fish samples were determined. Results showed that the trend of the ΔE value changes of the MAF/PVC film had a good correlation with the totla volatile basic nitrogen (TVB-N) content of the sample. Therefore, the freshness indicator based on MAF/PVC film could accurately and rapidly indicate the freshness of fish, thus showing a broad application prospect in detection of freshness of aquatic products.

Key words: Freshness indicator, Fish, Meldrum's activated furan

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