引用本文: 吴谦, 林花, 刘迎, 金永日, 黎鹏, 刘伟杰, 李绪文. 离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷. 分析化学, [复制]
Citation: WU Qian , LIN Hua , LIU Ying , JIN Yong-Ri , LI Peng , LIU Wei-Jie , XU Wen-Li . Ionic Liquid Ultrasonic-assisted with Solid Phase Extraction of Four kinds of Hosenkosides from the Seeds of Impatiens balsamina L.. Chinese Journal of Analytical Chemistry, [复制]
离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷
Ionic Liquid Ultrasonic-assisted with Solid Phase Extraction of Four kinds of Hosenkosides from the Seeds of Impatiens balsamina L.
本文建立了一种绿色、快速、高效萃取和富集中药急性子中4种凤仙萜四醇皂苷的方法。通过离子液体结合超声辅助萃取了急性子中的化学成分，并利用固相萃取小柱富集提取液中的凤仙萜四醇皂苷。通过离子液体种类、用量、pH值、超声时间、固相萃取剂和洗脱剂等实验条件对提取率影响的考察，确立了最优萃取剂为[C6mim]Br，最优萃取条件：萃取剂用量为0.9 mL，提取液pH=7，超声为30 min，最优富集条件：固相萃取小柱填料为0.9 g CHP20/P120，上样流速为1.5 mL/min，洗脱剂为1.5 mL甲醇。在最优条件下，4种凤仙萜四醇苷加标回收率在93.37%～108.18%之间，相关系数范围为0.9945～0.9975,检出限范围为1.79~4.55 μg/mL，RSD值均小于3.87%。该方法与传统回流提取方法提取效果接近，能准确测定急性子中4种凤仙萜四醇苷含量。
A green, rapid and efficient method was developed for the extraction of 4 hosenkosides from the seeds of Impatiens balsamina L. The hosenkosides were extracted by ionic liquid then enriched by solid phase extraction. The effects of the kinds and volume of ionic liquid, pH value, ultrasonic time, solid phase extractant and eluent on the extraction feilds were investigated eventually. Under the optimum conditions, the recoveries of 4 kinds of hosenkosides were between 93.37% and 108.18%, the correlation coefficients were in the rangs of 0.9945-0.9975, and the detection limits were 1.796~4.55 μg/mL. The RSD values were all less than 3.87%.The experimental results show that this method was fast, efficient, environmental protection. This paper provides a reference for the extraction and enrichment of trace components in Chinese medicinal herbs.
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离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷
Ionic Liquid Ultrasonic-assisted with Solid Phase Extraction of Four kinds of Hosenkosides from the Seeds of Impatiens balsamina L.
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